Eco-friendly decoration of graphene oxide with biogenic silver nanoparticles: antibacterial and antibiofilm activity

Journal of Nanoparticle Research volume 16, Article number: 2110 (2014) Cite this article

Abstract

This work reports on preparation, characterization, and antibacterial activity of graphene oxide (GO) decorated with biogenic silver nanoparticles (Bio-AgNPs) produced by the fungus Fusarium oxysporum. This nanocomposite (Bio-GOAg) was prepared by an ex situ process through the physical mixture of a GO dispersion with the previously prepared Bio-AgNPs. The adsorption of the Bio-AgNPs onto the GO sheets was confirmed by transmission electron microscopy. The average size of the Bio-AgNPs anchored onto the GO surface was found to be 3.5 nm. The antibacterial activity of the Bio-GOAg nanocomposite against Gram-positive and Gram-negative microorganisms was investigated and a very promising result was found for the Gram-negative strains. In addition, the Bio-GOAg nanocomposite displayed a very strong biocidal activity against the Salmonella typhimurium strain at a concentration of 2.0 μg/mL. The antibiofilm activity toward S. typhimurium adhered on stainless steel surfaces was also investigated. The results showed 100 % inhibition of the adhered cells after exposure to the Bio-GOAg nanocomposite for 1 h.

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Acknowledgments

A.F.F., P.D.M., D.S.T.M., A.C.M.M., N.D., and O.L.A. acknowledge the financial support of São Paulo State Research Foundation (FAPESP), National Council for Technological and Scientific Development (CNPq), National Institute of Science, Technology, and Innovation in Complex Functional Materials (INCT-Inomat), Brazilian Nanotoxicology Network (Cigenanotox), and NanoBioss-SisNano (MCTI). A.G.S.F. acknowledges the financial support of INCT NanoBioSimes (MCT-CNPq), Capes-Nanobiotec network, and Funcap through the grant Pronex (PR 2-0054-00022.01.00/11). A.B. acknowledges the financial support of CNPq and Capes. The authors acknowledge Prof.Amauri Jardim de Paula for his contributions to the transmission electron microscopy (TEM) analysis.

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Affiliations

  1. Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, Campinas, São Paulo, 13083-970, Brazil

    Andreia Fonseca de Faria, Ana Carolina Mazarin de Moraes, Priscyla Daniely Marcato, Diego Stéfani Teodoro Martinez, Nelson Durán & Oswaldo Luiz Alves

  2. School of Pharmaceutical Science of Ribeirão Preto, Universidade de São Paulo (USP), Ribeirão Preto, São Paulo, Brazil

    Priscyla Daniely Marcato

  3. Center of Natural and Human Sciences, Universidade Federal do ABC, Santo André, São Paulo, Brazil

    Nelson Durán

  4. Departamento de Física, Universidade Federal do Ceará, Caixa Postal 6030, Fortaleza, Ceará, 60455-900, Brazil

    Antônio Gomes Souza Filho

  5. Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Caixa Postal 15.090, Porto Alegre, Rio Grande do Sul, 91501-970, Brazil

    Adriano Brandelli

Authors
  1. Andreia Fonseca de Faria
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  2. Ana Carolina Mazarin de Moraes
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  3. Priscyla Daniely Marcato
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  4. Diego Stéfani Teodoro Martinez
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  5. Nelson Durán
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  6. Antônio Gomes Souza Filho
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  7. Adriano Brandelli
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  8. Oswaldo Luiz Alves
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Correspondence to Andreia Fonseca de Faria or Oswaldo Luiz Alves.

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de Faria, A.F., de Moraes, A.C.M., Marcato, P.D. et al. Eco-friendly decoration of graphene oxide with biogenic silver nanoparticles: antibacterial and antibiofilm activity. J Nanopart Res 16, 2110 (2014). https://doi.org/10.1007/s11051-013-2110-7

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Keywords

  • Graphene oxide
  • Biogenic silver nanoparticles
  • Fusarium oxysporum
  • Antibacterial activity
  • Salmonella typhimurium
  • Microbiology