Novel strontium-doped bioactive glass nanoparticles enhance proliferation and osteogenic differentiation of human bone marrow stromal cells
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The present study investigates a new family of bioactive glass nanoparticles with and without Sr-doping focusing on the influence of the nanoparticles on human bone marrow stromal cells (hBMSCs) in vitro. The bioactive glass nanoparticles were fabricated by flame spray synthesis and a particle diameter of 30–35 nm was achieved. Glass nanoparticles were undoped (BG 13-93-0Sr) or doped with 5 wt% strontium (Sr) (BG 13-93-5Sr) and used at concentrations of 10 and 100 μg/cm² (particles per culture plate area), respectively. Cells were cultured for 14 days after which the samples were analysed regarding metabolic activity and expression of various bone-specific genes. Cell growth and morphology indicated the high cytocompatibility of the nanoparticulate bioactive glass. The presence of the nanoparticles enhanced cell growth compared to the plain polystyrene control group. At a concentration of 100 μg/cm², Sr-doped particles led to significantly enhanced gene expression of osteocalcin, collagen type 1 and vascular endothelial growth factor. Thus, Sr-doped nanoparticles showing a dose-dependent increase of osteogenic differentiation in hBMSCs are a promising biomaterial for bone regeneration purposes.
KeywordsBioactive glass Human bone marrow stromal cells Nanoparticles Osteogenic differentiation Strontium Bone tissue engineering
This work was partially supported by the Emerging Fields Initiative (EFI) of the University of Erlangen-Nuremberg, Germany (project “TOPbiomat”), and a research grant from the Deutsche Forschungsgemeinschaft DFG (KN 578/2-1). The authors gratefully thank Marina Milde for expert technical assistance.
Conflict of interest
None of the authors has any financial interest to declare in relation to the content of this article.
- Alaiti MA, Ishikawa M, Masuda H, Simon DI, Jain MK, Asahara T, Costa MA (2012) Up-regulation of miR-210 by vascular endothelial growth factor in ex vivo expanded CD34+ cells enhances cell-mediated angiogenesis. J Cell Mol Med 16:2413–2421Google Scholar
- Arora P, Sindhu A, Dilbaghi N, Chaudhury A, Rajakumar G, Rahuman AA (2012) Nano-regenerative medicine towards clinical outcome of stem cell and tissue engineering in humans. J Cell Mol Med 16:1991–2000Google Scholar
- Gorustovich AA, Steimetz T, Cabrini RL, Porto Lopez JM (2010b) Osteoconductivity of strontium-doped bioactive glass particles: a histomorphometric study in rats. J Biomed Mater Res A 92:232–237Google Scholar
- Isaac J, Nohra J, Lao J, Jallot E, Nedelec JM, Berdal A, Sautier JM (2011) Effects of strontium-doped bioactive glass on the differentiation of cultured osteogenic cells. Eur Cell Mater 21:130–143Google Scholar
- Sila-Asna M, Bunyaratvej A, Maeda S, Kitaguchi H, Bunyaratavej N (2007) Osteoblast differentiation and bone formation gene expression in strontium-inducing bone marrow mesenchymal stem cell. Kobe J Med Sci 53:25–35Google Scholar