Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Developmental toxicity of CdTe QDs in zebrafish embryos and larvae

  • 569 Accesses

  • 12 Citations

Abstract

Quantum dots (QDs) have widely been used in biomedical and biotechnological applications. However, few studies focus on the assessing toxicity of QDs exposure in vivo. In this study, zebrafish embryos were treated with CdTe QDs (4 nm) during 4–96 h post-fertilization (hpf). Mortality, hatching rate, malformation, heart rate, and QDs uptake were detected. We also measured the larval behavior to analyze whether QDs had persistent effects on larvae locomotor activity at 144 hpf. The results showed that as the exposure dosages increased, the hatching rate and heart rate of zebrafish embryos were decreased, while the mortality increased. Exposure to QDs caused embryonic malformations, including head malformation, pericardial edema, yolk sac edema, bent spine, and yolk not depleted. QDs fluorescence was mainly localized in the intestines region. The larval behavior testing showed that the total swimming distance was decreased in a dose-dependent manner. The lowest dose (2.5 nM QDs) produced substantial hyperactivity while the higher doses groups (5, 10, and 20 nM QDs) elicited remarkably hypoactivity in dark periods. In summary, the data of this article indicated that QDs caused embryonic developmental toxicity, resulted in persistent effects on larval behavior.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

References

  1. Bar-Ilan O, Louis KM, Yang SP, Pedersen JA, Hamers RJ, Peterson RE, Heideman W (2012) Titanium dioxide nanoparticles produce phototoxicity in the developing zebrafish. Nanotoxicology 6:670–679

  2. Blechinger SR, Warren JT Jr, Kuwada JY, Krone PH (2002) Developmental toxicology of cadmium in living embryos of a stable transgenic zebrafish line. Environ Health Perspect 110:1041–1046

  3. Burgess HA, Granato M (2007) Modulation of locomotor activity in larval zebrafish during light adaptation. J Exp Biol 210:2526–2539

  4. Derfus AM, Chan WCW, Bhatia SN (2004) Probing the cytotoxicity of semiconductor quantum dots. Nano Lett 4:11–18

  5. Drapeau P, Saint-Amant L, Buss RR, Chong M, McDearmid JR, Brustein E (2002) Development of the locomotor network in zebrafish. Prog Neurobiol 68:85–111

  6. Duan JC, Yu YB, Li Y, Yu Y, Sun ZW (2013) Cardiovascular toxicity evaluation of silica nanoparticles in endothelial cells and zebrafish model. Biomater 34:5853–5862

  7. Fako VE, Furgeson DY (2009) Zebrafish as a correlative and predictive model for assessing biomaterial nanotoxicity. Adv Drug Deliv Rev 61:478–486

  8. Flanagan-Steet H, Fox MA, Meyer D, Sanes JR (2005) Neuromuscular synapses can form in vivo by incorporation of initially a neural postsynaptic specializations. Development 132(20):4471–4481

  9. Gao X, Yin S, Tang M, Chen J, Yang Z, Zhang W, Chen L, Yang B, Li Z, Zha Y, Ruan D, Wang M (2011) Effects of developmental exposure to TiO2 nanoparticles on synaptic plasticity in hippocampal dentate gyrus area: an in vivo study in anesthetized rats. Biol Trace Elem Res 143:1616–1628

  10. Glickman NS, Yelon D (2002) Cardiac development in zebrafish: coordination of form and function. Semin Cell Dev Biol 13:507–513

  11. Han Y, Xie G, Sun Z, Mu Y, Han S, Xiao Y, Liu N, Wang H, Guo C, Shi Z (2011) Plasma kinetics and biodistribution of water-soluble CdTe quantum dots in mice: a comparison between Cd and Te. J Nanopart Res 13:5373–5380

  12. Hecker L, Khait L, Sessions SK, Birla RK (2008) Functional evaluation of isolated zebrafish hearts. Zebrafish 5:319–322

  13. Heiden TC, Dengler E, Kao WJ, Heideman W, Peterson RE (2007) Developmental toxicity of low generation PAMAM dendrimers in zebrafish. Toxicol Appl Pharmacol 225:70–79

  14. Hen Chow ES, Cheng SH (2003) Cadmium affects muscle type development and axon growth in zebrafish embryonic somitogenesis. Toxicol Sci 73:149–159

  15. Hill AJ, Teraoka H, Heideman W, Peterson RE (2005) Zebrafish as a model vertebrate for investigating chemical toxicity. Toxicol Sci 86:6–19

  16. Irons TD, MacPhail RC, Hunter DL, Padilla S (2010) Acute neuroactive drug exposures alter locomotor activity in larval zebrafish. Neurotoxicol Teratol 32:84–90

  17. King-Heiden TC, Wiecinski PN, Mangham AN, Metz KM, Nesbit D, Pedersen JA, Hamers RJ, Heideman W, Peterson RE (2009) Quantum dot nanotoxicity assessment using the zebrafish embryo. Environ Sci Technol 43:1605–1611

  18. Lee KJ, Browning LM, Nallathamby PD, Desai T, Cherukuri PK, Xu XH (2012a) In vivo quantitative study of sized-dependent transport and toxicity of single silver nanoparticles using zebrafish embryos. Chem Res Toxicol 25:1029–1046

  19. Lee KJ, Nallathamby PD, Browning LM, Desai T, Cherukuri PK, Xu XH (2012b) Single nanoparticle spectroscopy for real-time in vivo quantitative analysis of transport and toxicity of single nanoparticles in single embryos. Analyst 137:2973–2986

  20. Levin ED, Aschner M, Heberlein U, Ruden D, Welsh-Bohmer KA, Bartlett S, Berger K, Chen L, Corl AB, Eddins D, French R, Hayden KM, Helmcke K, Hirsch HV, Linney E, Lnenicka G, Page GP, Possidente D, Possidente B, Kirshner A (2009) Genetic aspects of behavioral neurotoxicology. Neurotoxicology 30(5):741–753

  21. Lewinski NA, Zhu H, Ouyang CR, Conner GP, Wagner DS, Colvin VL, Drezek RA (2011) Trophic transfer of amphiphilic polymer coated CdSe/ZnS quantum dots to Danio rerio. Nanoscale 3:3080–3083

  22. Lieschke GJ, Currie PD (2007) Animal models of human disease: zebrafish swim into view. Nat Rev Genet 8:353–367

  23. Lin S, Zhao Y, Xia T, Meng H, Ji Z, Liu R, George S, Xiong S, Wang X, Zhang H, Pokhrel S, Madler L, Damoiseaux R, Nel AE (2011) High content screening in zebrafish speeds up hazard ranking of transition metal oxide nanoparticles. ACS Nano 5:7284–7295

  24. Luna VM, Brehm P (2006) An electrically coupled network of skeletal muscle in zebrafish distributes synaptic current. J Gen Physiol 128(1):89–102

  25. MacPhail RC, Brooks J, Hunter DL, Padnos B, Irons TD, Padilla S (2009) Locomotion in larval zebrafish: influence of time of day, lighting and ethanol. Neurotoxicology 30:52–58

  26. Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, Sundaresan G, Wu AM, Gambhir SS, Weiss S (2005) Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307:538–544

  27. Mitra RN, Doshi M, Zhang X, Tyus JC, Bengtsson N, Fletcher S, Page BD, Turkson J, Gesquiere AJ, Gunning PT, Walter GA, Santra S (2012) An activatable multimodal/multifunctional nanoprobe for direct imaging of intracellular drug delivery. Biomaterials 33:1500–1508

  28. Powers CM, Badireddy AR, Ryde IT, Seidler FJ, Slotkin TA (2011) Silver nanoparticles compromise neurodevelopment in PC12 cells: critical contributions of silver ion, particle size, coating, and composition. Environ Health Perspect 119:37–44

  29. Prober DA, Rihel J, Onah AA, Sung RJ, Schier AF (2006) Hypocretin/orexin overexpression induces an insomnia-like phenotype in zebrafish. J Neurosci 26:13400–13410

  30. Pyati UJ, Look AT, Hammerschmidt M (2007) Zebrafish as a powerful vertebrate model system for in vivo studies of cell death. In: Seminars in cancer biology, Elsevier, Amsterdam, pp 154–165

  31. Riehl R, Kyzar E, Allain A, Green J, Hook M, Monnig L, Rhymes K, Roth A, Pham M, Razavi R, Dileo J, Gaikwad S, Hart P, Kalueff AV (2011) Behavioral and physiological effects of acute ketamine exposure in adult zebrafish. Neurotoxicol Teratol 33:658–667

  32. Ruan J, Song H, Qian Q, Li C, Wang K, Bao C, Cui D (2012) HER2 monoclonal antibody conjugated RNase-A-associated CdTe quantum dots for targeted imaging and therapy of gastric cancer. Biomaterials 33:7093–7102

  33. Tang M, Wang M, Xing T, Zeng J, Wang H, Ruan DY (2008) Mechanisms of unmodified CdSe quantum dot-induced elevation of cytoplasmic calcium levels in primary cultures of rat hippocampal neurons. Biomaterials 29:4383–4391

  34. Truong L, Saili KS, Miller JM, Hutchison JE, Tanguay RL (2012) Persistent adult zebrafish behavioral deficits results from acute embryonic exposure to gold nanoparticles. Comp Biochem Physiol C Toxicol Pharmacol 155:269–274

  35. Usenko CY, Harper SL, Tanguay RL (2007) In vivo evaluation of carbon fullerene toxicity using embryonic zebrafish. Carbon N Y 45:1891–1898

  36. Verkerk AO, Remme CA (2012) Zebrafish: a novel research tool for cardiac (patho)electrophysiology and ion channel disorders. Front Physiol 3:255

  37. Wagner DS, Delk NA, Lukianova-Hleb EY, Hafner JH, Farach-Carson MC, Lapotko DO (2010) The in vivo performance of plasmonic nanobubbles as cell theranostic agents in zebrafish hosting prostate cancer xenografts. Biomaterials 31:7567–7574

  38. Woodward JD, Kennel SJ, Mirzadeh S, Dai S, Wall JS, Richey T, Avenell J, Rondinone AJ (2007) In vivo SPECT/CT imaging and biodistribution using radioactive Cd125mTe/ZnS nanoparticles. Nanotechnology 18:175103

  39. Xu Z, Zhang YL, Song C, Wu LL, Gao HW (2012) Interactions of hydroxyapatite with proteins and its toxicological effect to zebrafish embryos development. PLoS ONE 7:e32818

  40. Yan Y, Mu Y, Feng G, Zhang L, Zhu L, Xu L, Yang R, Jin Q (2008) Novel strategy for synthesis of high quality CdTe nanocrystals in aqueous solution. Chem Res Chin Univ 24:8–14

  41. Yan H, Teh C, Sreejith S, Zhu L, Kwok A, Fang W, Ma X, Nguyen KT, Korzh V, Zhao Y (2012) Functional mesoporous silica nanoparticles for photothermal-controlled drug delivery in vivo. Angew Chem Int Ed Engl 51:8373–8377

  42. Yang RSH, Chang LW, Wu JP, Tsai MH, Wang HJ, Kuo YC, Yeh TK, Yang CS, Lin P (2007) Persistent tissue kinetics and redistribution of nanoparticles, quantum dot 705, in mice: ICP-MS quantitative assessment. Environ Health Perspect 115:1339

  43. Zhang T, Stilwell JL, Gerion D, Ding L, Elboudwarej O, Cooke PA, Gray JW, Alivisatos AP, Chen FF (2006) Cellular effect of high doses of silica-coated quantum dot profiled with high throughput gene expression analysis and high content cellomics measurements. Nano Lett 6:800–808

  44. Zhang W, Lin K, Miao Y, Dong Q, Huang C, Wang H, Guo M, Cui X (2012) Toxicity assessment of zebrafish following exposure to CdTe QDs. J Hazard Mater 213–214:413–420

  45. Zhu X, Zhu L, Duan Z, Qi R, Li Y, Lang Y (2008) Comparative toxicity of several metal oxide nanoparticle aqueous suspensions to Zebrafish (Danio rerio) early developmental stage. J Environ Sci Health A 43:278–284

  46. Zhu ZJ, Carboni R, Quercio MJ Jr, Yan B, Miranda OR, Anderton DL, Arcaro KF, Rotello VM, Vachet RW (2010) Surface properties dictate uptake, distribution, excretion, and toxicity of nanoparticles in fish. Small 6:2261–2265

Download references

Acknowledgments

The authors would like to thank Prof. Ying Mu from Zhejiang University for the preparation of CdTe QDs. This study was supported by the National Natural Science Foundation of China (Nos. 81230065 and 81172704), the Funding Project for Academic Human Resources Development of Beijing Education Committee (PHR201006110), and the Innovative Team Project of Beijing Education Committee (PHR201107116).

Author information

Correspondence to Shuangqing Peng or Zhiwei Sun.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Duan, J., Yu, Y., Li, Y. et al. Developmental toxicity of CdTe QDs in zebrafish embryos and larvae. J Nanopart Res 15, 1700 (2013). https://doi.org/10.1007/s11051-013-1700-8

Download citation

Keywords

  • Quantum dots (QDs)
  • Zebrafish
  • Embryonic developmental toxicity
  • QDs uptake
  • Larval behavior