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A Promising Combo Gene Delivery System Developed from (3-Aminopropyl)triethoxysilane-Modified Iron Oxide Nanoparticles and Cationic Polymers

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Abstract

(3-Aminopropyl)triethoxysilane-modified iron oxide nanoparticles (APTES-IONPs) have been evaluated for various biomedical applications, including medical imaging and drug delivery. Cationic polymers (CPs) such as Lipofectamine and TurboFect are widely used for research in gene delivery, but their toxicity and low in vivo efficiency limited their further application. In the present study, we synthesized water-soluble APTES-IONPs and developed a combo gene delivery system based on APTES-IONPs and CPs. This system significantly increased gene-binding capacity, protected genes from degradation, and improved gene transfection efficiency for DNA and siRNA in both adherent and suspension cells. Because of its great biocompatibility, high gene-carrying ability, and very low cytotoxicity, this combo gene delivery system will be expected for a wide application, and it might provide a new method for gene therapy.

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Acknowledgments

This project was supported by the National Basic Research Program of China (Program “973”, 2011CB933501), by the National Natural Science Foundation of China (Grants No. 81071935, 81272632, 81101795), by the Natural Science Foundation of Jiangsu Province (Grants No. BK2010218, BK2011268), and by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Cyrus Tang Hematology Center, Soochow University, 199 Ren Ai Road, Room 703-507, Suzhou Industrial Park, Suzhou, 215123, China

    Zubin Zhang, Xiaolin Du, Biyin Cao & Xinliang Mao

  2. State Key Laboratory of Bioelectronics and Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science & Medical Engineering, Southeast University, Nanjing, 210096, China

    Lina Song, Jinlai Dong, Dawei Guo, Yu Zhang & Ning Gu

Authors
  1. Zubin Zhang
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  2. Lina Song
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  3. Jinlai Dong
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  4. Dawei Guo
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  5. Xiaolin Du
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  6. Biyin Cao
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  7. Yu Zhang
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  8. Ning Gu
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  9. Xinliang Mao
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Correspondence to Xinliang Mao.

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Zhang, Z., Song, L., Dong, J. et al. A Promising Combo Gene Delivery System Developed from (3-Aminopropyl)triethoxysilane-Modified Iron Oxide Nanoparticles and Cationic Polymers. J Nanopart Res 15, 1659 (2013). https://doi.org/10.1007/s11051-013-1659-5

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  • DOI: https://doi.org/10.1007/s11051-013-1659-5

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