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In vitro and in vivo investigations on bone regeneration potential of laminated hydroxyapatite/gelatin nanocomposite scaffold along with DBM

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Abstract

Bone regeneration ability of a scaffold strongly depends on its structure and the size of its components. In this study, a nanostructured scaffold was designed for bone repair using nano hydroxyapatite (nHA) (8–16 nm × 50–80 nm) and gelatin (GEL) as main components. In vitro investigations of calcium matrix deposition and gene expression of the seeded cells for this scaffold, demineralized bone matrix (DBM), scaffold plus DBM, and the control group were carried out. Bone regeneration in rat calvarium with critical defect size after 1, 4, and 8 weeks post implantation was investigated. The calcium matrix depositions by the osteoblast and RUNX2, ALP, osteonectin, and osteocalcin gene expression in scaffold were more significant than in other groups. Histomorphometry analysis confirmed in vitro results. In vitro and in vivo bone regeneration were least in scaffold plus DBM group. Enhanced effects in scaffold could be attributed to the shape and size of nHA particles and good architecture of the scaffold. Reduction of bone regeneration might be due to tight bonding of BMPs and nHA particles in the third group. Results obtained from this study confirmed that nano-scale size of the main components and the scaffold architecture (pore diameter, interconnectivity pores, etc.) have significant effects on bone regeneration ability of the scaffold and are important parameters in designing a temporary bone substitute.

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Acknowledgments

This study was supported by grant from the School of Advanced Medical Technologies, the Tehran University of Medical Sciences. The authors thank the Iranian Tissue Bank Research & Preparation Center of Imam Khomeini Medical Hospital Complex for supplying DBM.

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Authors and Affiliations

  1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, 1417755469, Tehran, Iran

    Shima Tavakol, Sharmin Kharrazi & Seyed Mahdi Rezayat Sorkhabadi

  2. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Iraj Ragerdi Kashani

  3. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Mahmood Azami

  4. Iranian Tissue Bank Research & Preparation Center, Tehran University of Medical Sciences, Tehran, Iran

    Ahad Khoshzaban

  5. Department of Medicine, Kashan University of Medical Sciences, Kashan, Iran

    Behnaz Tavakol

  6. Department of Biology, Faculty of Sciences, University of Tarbiat Moallem, Tehran, Iran

    Somayeh Ebrahimi

  7. Department of Toxicology & Pharmacology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran

    Seyed Mahdi Rezayat Sorkhabadi

  8. Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Seyed Mahdi Rezayat Sorkhabadi

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  1. Shima Tavakol
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  2. Iraj Ragerdi Kashani
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  3. Mahmood Azami
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  4. Ahad Khoshzaban
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  5. Behnaz Tavakol
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  7. Somayeh Ebrahimi
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  8. Seyed Mahdi Rezayat Sorkhabadi
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Correspondence to Seyed Mahdi Rezayat Sorkhabadi.

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Tavakol, S., Ragerdi Kashani, I., Azami, M. et al. In vitro and in vivo investigations on bone regeneration potential of laminated hydroxyapatite/gelatin nanocomposite scaffold along with DBM. J Nanopart Res 14, 1265 (2012). https://doi.org/10.1007/s11051-012-1265-y

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  • DOI: https://doi.org/10.1007/s11051-012-1265-y

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