Abstract
Room temperature biosynthesis of Ag, Pd, Fe, Rh, Ni, Ru, Pt, Co, and Li nanoparticles was achieved using Pseudomonas aeruginosa SM1 without the addition of growth media, electron donors, stabilizing agents, preparation of cell/cell-free extract or temperature, and pH adjustments. The resulting nanoparticles were characterized by Transmission electron microscopy and X-ray diffraction. It was observed that P. aeruginosa SM1 is capable of producing both intracellular (Co and Li) and extracellular (Ag, Pd, Fe, Rh, Ni, Ru, and Pt) nanoparticles in both crystalline and amorphous state. The FT-IR spectra clearly showed the presence of primary and secondary amines which may be responsible for the reduction and subsequent stabilization of the resulting extracellular nanoparticles which were obtained as a one-step process. This suggests toward an unknown “selection mechanism” that reduces certain metal ions and allows others to enter the cell membrane. Finally, in this first of its kind study, single strain of bacteria was used to produce several different mono-metallic nanoparticles.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Artemov D, Mori N, Okollie B, Bhujwalla ZM (2003) MR molecular imaging of the Her-2/neu receptor in breast cancer cells using targeted iron oxide nanoparticles. Magn Reson Med 49:403–408
Bhambure R, Bule M, Shaligram N, Kamat M, Singhal R (2009) Extracellular biosynthesis of gold nanoparticles using Aspergillus niger—its characterization and stability. Chem Eng Technol 32:1036–1041
Blanche F, Couder M, Debussche L, Thibaut D, Cameron B, Crouzet J (1991) Biosynthesis of vitamin B12: stepwise amidation of carboxyl groups b, d, e, and g of cobyrinic acid a,c-diamide is catalyzed by one enzyme in Pseudomonas denitrificans. J Bacteriol 173:6046–6051
Bruins RM, Kapil S, Oehme SW (2000) Microbial resistance to metals in the environment. Ecotoxicol Environ Saf 45:198–207
Craig BD, Anderson DS (1995) Atmospheric environment: handbook of corrosion data. ASM International p 126
Cui H, Zayat M, Levy D (2005) Nanoparticle synthesis of willemite doped with cobalt ions (Co0.05Zn1.95SiO4) by an epoxide-assisted sol–gel method. Chem Mater 17:5562–5566
De Windt D, Aelterman P, Verstraete W (2005) Bioreductive deposition of palladium (0) nanoparticles on Shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls. Environ Microbiol 7:314–325
Gopidas KR, Whitesell JK, Fox MA (2003) Synthesis, characterization, and catalytic applications of a palladium-nanoparticle-cored dendrimer. Nano Lett 3:1757–1760
Hallmann J, Hallmann AQ, Mahaffee WF, Kloepper JW (1997) Bacterial endophytes in agricultural crops. Can J Microbiol 43:895–914
Huber DL (2005) Synthesis, properties, and applications of iron nanoparticles. Small 1:482–501
Husseiny MI, Abd El-Aziz M, Badr Y, Mahmoud MA (2007) Biosynthesis of gold nanoparticles using Pseudomonas aeruginosa. Spectrochim Acta A 67:1003–1006
Joerger R, Klaus T, Granqvist CG (2000) Biologically produced silver-carbon composite materials for optically functional thin-film coatings. Adv Mater 12:407–409
Kima TR, Kima DH, Ryua HW, Moona JH, Leeb JH, Boob S, Kim J (2007) Synthesis of lithium manganese phosphate nanoparticle and its properties. J Phys Chem Solids 68:1203–1206
Klaus-Joerger T, Joerger R, Olsson E, Granqvist CG (2001) Bacteria as workers in the living factory: metal-accumulating bacteria and their potential for materials science. Trends Biotechnol 19:15–20
Konishi Y, Tsukiyama T, Tachimi T, Saitoh N, Nomura T, Nagamine S (2007) Microbial deposition of gold nanoparticles by the metal-reducing bacterium Shewanella algae. Electrochim Acta 53:186–192
Kuroda T, Fujita N, Utsugi J, Kuroda M, Mizushima T, Tsuchiya T (2004) A major Li(+) extrusion system NhaB of Pseudomonas aeruginosa: comparison with the major Na(+) extrusion system NhaP. Microbiol Immunol 48:243–250
Lengke M, Southam G (2006) Bioaccumulation of gold by sulfate-reducing bacteria cultured in the presence of gold(I)-thiosulfate complex. Geochim Cosmochim Acta 70:3646–3661
Lide I, David R (2007) Platinum. CRC handbook of chemistry and physics, 4th edn. CRC Press, New York
Liu Z, Ling XY, Su X, Lee JY (2004) Carbon-supported Pt and PtRu nanoparticles as catalysts for a direct methanol fuel cell. J Phys Chem B 108:8234–8240
Mann S, Frankel RB, Blakemore RP (1984) Structure, morphology, and crystal growth of bacterial magnetite. Nature 310:405–407
Molenbroek AM, Nørskov JK (2001) Structure and reactivity of Ni–Au nanoparticle catalysts. J Phys Chem B 105:5450–5458
Mu XD, Meng JQ, Li ZC, Kou Y (2005) Rhodium nanoparticles stabilized by ionic copolymers in ionic liquids: long lifetime nanocluster catalysts for benzene hydrogenation. J Am Chem Soc 127:9694–9695
Ogi T, Saitoh N, Nomura T, Konishi Y (2010) Room-temperature synthesis of gold nanoparticles and nanoplates using Shewanella algae cell extract. J Nanopart Res 12:2531–2539
Pugazhenthiran N, Anandan S, Kathiravan G, Prakash NKU, Crawford S, Ashokkumar MJ (2009) Microbial synthesis of silver nanoparticles by Bacillus sp. J Nanopart Res 11:1811–1815
Rai M, Yadava A, Gadea A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27:76–83
Riddina T, Gerickeb M, Whiteleya CG (2010) Biological synthesis of platinum nanoparticles: effect of initial metal concentration. Enzyme Microbiol Technol 46:501–505
Silverstein RM, Webster FX, Kiemle D (2005) Spectrometric identification of organic compounds, 7th edn. Wiley, New York, pp 101–108
Son WK, Youk JH, Lee TS, Park WH (2004) Preparation of antimicrobial ultrafine cellulose acetate fibers with silver nanoparticles. Macromol Rapid Commun 25:1632–1637
Yong P, Rowsen NA, Farr JPG, Harris IR, Macaskie LE (2002) Bioreduction and biocrystallization of palladium by Desulfovibrio desulfuricans. Biotechnol Bioeng 80:369–379
Zhang X, Chan KY (2003) Water-in-oil microemulsion synthesis of platinum–ruthenium nanoparticles, their characterization and electrocatalytic properties. Chem Mater 15:451–459
Zhang H, Li Q, Lu Y, Sun D, Lin X, Deng X et al (2005) Biosorption and bioreduction of diamine silver complex by Corynebacterium. J Chem Technol Biotechnol 80:285–290
Zhang X, Yan S, Tyagi RD, Surampalli RY (2011) Synthesis of nanoparticles by microorganisms and their application in enhancing microbiological reaction rates. Chemosphere 82:489–494
Acknowledgments
We would like to thank the Spanish Ministry of Science and Innovation for its financial support through the Grant BIO2008-02841.
Our gratitude also goes to Dr. Francesc Gispert Guirado of the Scientific and Technical Resources Service of the URV (Tarragona) for his help with the X-ray diffraction analyses.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Srivastava, S.K., Constanti, M. Room temperature biogenic synthesis of multiple nanoparticles (Ag, Pd, Fe, Rh, Ni, Ru, Pt, Co, and Li) by Pseudomonas aeruginosa SM1. J Nanopart Res 14, 831 (2012). https://doi.org/10.1007/s11051-012-0831-7
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s11051-012-0831-7