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Comparison of the photosensitivity and bacterial toxicity of spherical and tubular fullerenes of variable aggregate size

Abstract

Nanomaterials such as fullerene C60, carbon nanotubes (CNTs), and other fullerenes show unique electrical, chemical, mechanical, and thermal properties that are not well understood in the context of the environmental behavior of this class of carbon-based materials. In this study, aqueous suspensions of three fullerenes nanoparticles, C60, single-wall (SW) and multi-wall (MW) CNTs were prepared by sonication and tested for reactive oxygen species (ROS) production and inactivation of Vibrio fischeri, a gram-negative rod-shaped bacterium, under ultraviolet (UV)-A irradiation. We show that ROS production and microbial inactivation increases as colloidal aggregates of C60, SWCNT, and MWCNT are fractionated to enrich with smaller aggregates by progressive membrane filtration. As the quantity and influence of these more reactive fractions of the suspension may increase with time and/or as the result of fractionation processes in the laboratory or the environment, experiments evaluating photo-reactivity and toxicity endpoints must take into account the evolution and heterogeneity of nanoparticle aggregates in water.

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References

  • Agnihotri S, Mota JPB, Rostam-Abadi M, Rood MJ (2005) Structural characterization of single-walled carbon nanotube bundles by experiment and molecular simulation. Langmuir 21:896–904

    Article  CAS  Google Scholar 

  • Allen BL, Kichambare PD, Star A (2007) Carbon nanotube field-effect-transistor-based biosensors. Adv Mater 19:1439–1451

    Article  CAS  Google Scholar 

  • Ball P (2001) Roll up for the revolution. Nature 414:142–144

    Article  CAS  Google Scholar 

  • Brady-Estevez AS, Kang S, Elimelech M (2008) A single-walled-carbon-nanotube filter for removal of viral and bacterial pathogens. Small 4:481–484

    Article  CAS  Google Scholar 

  • Brunet L, Lyon DY, Zodrow K, Rouch JC, Caussat B, Serp P, Remigy JC, Wiesner MR, Alvarez PJJ (2008) Properties of membranes containing semi-dispersed carbon nanotubes. Environ Eng Sci 25:565–575

    Article  CAS  Google Scholar 

  • Chae SR, Hotze EM, Wiesner MR (2009a) Evaluation of the oxidation of organic compounds by aqueous suspensions of photosensitized hydroxylated-C-60 fullerene aggregates. Environ Sci Technol 43:6208–6213

    Article  CAS  Google Scholar 

  • Chae SR, Wang SY, Hendren ZD, Wiesner MR, Watanabe Y, Gunsch CK (2009b) Effects of fullerene nanoparticles on Escherichia coli K12 respiratory activity in aqueous suspension and potential use for membrane biofouling control. J Membr Sci 329:68–74

    Article  CAS  Google Scholar 

  • Chae SR, Badireddy AR, Budarz JF, Lin S, Xiao Y, Therezien M, Wiesner MR (2010) Heterogeneities in fullerene nanoparticle aggregates affecting reactivity, bioactivity, and transport. ACS Nano 4:5011–5018

    Article  CAS  Google Scholar 

  • Chae SR, Watanabe Y, Wiesner MR (2011) Comparative photochemical reactivity of spherical and tubular fullerene nanoparticles in water under ultraviolet (UV) irradiation. Water Res 45:308–314

    Article  CAS  Google Scholar 

  • Chen W, Tao XM, Xue P, Cheng XY (2005) Enhanced mechanical properties and morphological characterizations of poly(vinyl alcohol)-carbon nanotube composite films. Appl Surf Sci 252:1404–1409

    Article  CAS  Google Scholar 

  • Gryglik D, Miller JS, Ledakowicz S (2007) Singlet molecular oxygen application for 2-chlorophenol removal. J Hazard Mater 146:502–507

    Article  CAS  Google Scholar 

  • Hotze EM, Labille J, Alvarez P, Wiesner MR (2008) Mechanisms of photochemistry and reactive oxygen production by fullerene suspensions in water. Environ Sci Technol 42:4175–4180

    Article  CAS  Google Scholar 

  • Jassby D, Chae SR, Hendren Z, Wiesner M (2010) Membrane filtration of fullerene nanoparticle suspensions: Effects of derivatization, pressure, electrolyte species and concentration. J Colloid Interface Sci 346:296–302

    Article  CAS  Google Scholar 

  • Kang S, Pinault M, Pfefferle LD, Elimelech M (2007) Single-walled carbon nanotubes exhibit strong antimicrobial activity. Langmuir 23:8670–8673

    Article  CAS  Google Scholar 

  • Kang S, Herzberg M, Rodrigues DF, Elimelech M (2008a) Antibacterial effects of carbon nanotubes: size does matter. Langmuir 24:6409–6413

    Article  CAS  Google Scholar 

  • Kang S, Mauter MS, Elimelech M (2008b) Physicochemical determinants of multiwalled carbon nanotube bacterial cytotoxicity. Environ Sci Technol 42:7528–7534

    Article  CAS  Google Scholar 

  • Kateb B, Van Handel M, Zhang LY, Bronikowski MJ, Manohara H, Badie B (2007) Internalization of MWCNTs by microglia: possible application in immunotherapy of brain tumors. Neuroimage 37:S9–S17

    Article  Google Scholar 

  • Lam CW, James JT, McCluskey R, Arepalli S, Hunter RL (2006) A review of carbon nanotube toxicity and assessment of potential occupational and environmental health risks. Crit Rev Toxicol 36:189–217

    Article  CAS  Google Scholar 

  • Lee J, Fortner JD, Hughes JB, Kim JH (2007) Photochemical production of reactive oxygen species by C-60 in the aqueous phase during UV irradiation. Environ Sci Technol 41:2529–2535

    Article  CAS  Google Scholar 

  • Mauter MS, Elimelech M (2008) Environmental applications of carbon-based nanomaterials. Environ Sci Technol 42:5843–5859

    Article  CAS  Google Scholar 

  • Maynard AD, Aitken RJ, Butz T, Colvin V, Donaldson K, Oberdorster G, Philbert MA, Ryan J, Seaton A, Stone V, Tinkle SS, Tran L, Walker NJ, Warheit DB (2006) Safe handling of nanotechnology. Nature 444:267–269

    Article  CAS  Google Scholar 

  • McConkey BJ, Duxbury CL, Dixon DG, Greenberg BM (1997) Toxicity of a PAH photooxidation product to the bacteria Photobacterium phosphoreum and the duckweed Lemna gibba: effects of phenanthrene and its primary photoproduct, phenanthrenequinone. Environ Toxicol Chem 16:892–899

    CAS  Google Scholar 

  • Mueller NC, Nowack B (2008) Exposure modeling of engineered nanoparticles in the environment. Environ Sci Technol 42:4447–4453

    Article  CAS  Google Scholar 

  • Robichaud CO, Tanzil D, Weilenmann U, Wiesner MR (2005) Relative risk analysis of several manufactured nanomaterials: an insurance industry context. Environ Sci Technol 39:8985–8994

    Article  CAS  Google Scholar 

  • Veetil JV, Ye KM (2009) Tailored carbon nanotubes for tissue engineering applications. Biotechnol Prog 25:709–721

    Article  CAS  Google Scholar 

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Acknowledgments

This material is based upon work supported by the National Science Foundation (NSF) and the Environmental Protection Agency (EPA) under NSF Cooperative Agreement EF-0830093, Center for the Environmental Implications of NanoTechnology (CEINT). Any opinions, findings, conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the NSF or the EPA. This study has not been subjected to EPA review and no official endorsement should be inferred.

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Correspondence to So-Ryong Chae.

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Chae, SR., Therezien, M., Budarz, J.F. et al. Comparison of the photosensitivity and bacterial toxicity of spherical and tubular fullerenes of variable aggregate size. J Nanopart Res 13, 5121 (2011). https://doi.org/10.1007/s11051-011-0492-y

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  • DOI: https://doi.org/10.1007/s11051-011-0492-y

Keywords

  • Fullerene
  • Carbon nanotubes
  • Reactive oxygen species (ROS)
  • Vibrio fischeri
  • Colloidal aggregates
  • Heterogeneity
  • Environmental effects
  • EHS