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VEGFR-2 targeted cellular delivery of doxorubicin by gold nanoparticles for potential antiangiogenic therapy

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Abstract

We report a novel gold nanobioconjugate system that achieves targeted delivery of the small molecule drug doxorubicin to endothelial cells using anti-VEGFR-2 antibody conjugated gold nanoparticles (GNPs). The reported nanobioconjugate system combines the inherent ability of GNPs to undergo high levels of derivatization with the precision of antibody recognition of a cell surface antigen. Transmission electron microscopy (TEM) and surface-enhanced Raman spectroscopy (SERS) confirmed intracellular presence of the GNPs. Using a VEGFR-2 expressing cell line and a cell line that is negative for the receptor, in combination with competition assay we established the cell specific targeted delivery of the nanobioconjugate. The nanobioconjugate system described here may have potential drug delivery applications for antiangiogenic cancer therapy.

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Acknowledgments

This work was supported by a start-up fund from Kent State University to SB. AH was supported by the National Science Foundation through an NSF-REU summer fellowship.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Kent State University, Kent, OH, 44242, USA

    Arijit Das, Eric Soehnlen, Stephan Woods, Ravi Hegde, Amanda Henry, Arne Gericke & Soumitra Basu

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  1. Arijit Das
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  2. Eric Soehnlen
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  3. Stephan Woods
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  4. Ravi Hegde
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  5. Amanda Henry
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  6. Arne Gericke
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  7. Soumitra Basu
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Correspondence to Soumitra Basu.

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Das, A., Soehnlen, E., Woods, S. et al. VEGFR-2 targeted cellular delivery of doxorubicin by gold nanoparticles for potential antiangiogenic therapy. J Nanopart Res 13, 6283–6290 (2011). https://doi.org/10.1007/s11051-011-0300-8

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  • DOI: https://doi.org/10.1007/s11051-011-0300-8

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