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Synthesis and characterization of bovine femur bone hydroxyapatite containing silver nanoparticles for the biomedical applications

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Abstract

Bovine femur bone hydroxyapatite (HA) containing silver (Ag) nanoparticles was synthesized by thermal decomposition method and subsequent reduction of silver nitrate with N,N-dimethylformamide (DMF) in the presence of poly(vinylacetate) (PVAc). The structural, morphological, and chemical properties of the HA–Ag nanoparticles were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), field-emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). TEM images showed that the Ag nanoparticles with size ranging from 8 to 20 nm and were arranged at the periphery of HA crystals. Bactericidal activity of HA–Ag with different concentration of Ag nanoparticles immobilized on the surface of HA was investigated against gram-positive Staphylococcus aureus (S. aureus, non-MRSA), Methicillin resistant S. aureus (MRSA) and gram-negative Escherichia coli (E. coli) by the disc diffusion susceptibility test. The HA–Ag nanoparticles showed that broad spectrum activity against non-MRSA, MRSA, and E. coli bacterial strains.

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Acknowledgments

This study was supported by the Korean Research Foundation Grant Funded by Korea Government (MOEHRD; KRF–2005-210-D00042) and Regional Research Centers Programs of the Korean Ministry of Education and Human Resource Development through the Center for Healthcare Technology Development. One of the authors RN sincerely acknowledges the help provided by Dr. Atul A. Chaudhri, and Ms. In Hee, College of Veterinary Medicine, Chonbuk National University and Dr. Nasser A. M. Barakat, Chemical Engineering Department, El-Minia University, Egypt for his valuable suggestions.

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Authors and Affiliations

  1. Bio-Nano System Engineering, College of Engineering, Chonbuk National University, Deokjin-dong 664-14, Jeonju, 561-756, South Korea

    R. Nirmala & Faheem A. Sheikh

  2. Department of Polymer Nano Science and Technology, Chonbuk National University, Jeonju, 561-756, South Korea

    Muzafar A. Kanjwal

  3. College of Veterinary Medicine, Chonbuk National University, Deokjin-dong 664-14, Jeonju, 561-756, South Korea

    John Hwa Lee

  4. Department of Organic Materials and Fiber Engineering, Center for Healthcare Technology and Development, Chonbuk National University, Deokjin-dong 664-14, Jeonju, 561-756, South Korea

    Soo-Jin Park & Hak Yong Kim

  5. Semiconductor Materials Processing Laboratory, School of Advanced Materials Engineering, College of Engineering, Chonbuk National University, Deokjin-dong 664-14, Jeonju, 561-756, South Korea

    R. Navamathavan

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  1. R. Nirmala
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  2. Faheem A. Sheikh
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Correspondence to Hak Yong Kim.

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Nirmala, R., Sheikh, F.A., Kanjwal, M.A. et al. Synthesis and characterization of bovine femur bone hydroxyapatite containing silver nanoparticles for the biomedical applications. J Nanopart Res 13, 1917–1927 (2011). https://doi.org/10.1007/s11051-010-9944-z

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  • DOI: https://doi.org/10.1007/s11051-010-9944-z

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