Journal of Nanoparticle Research

, Volume 12, Issue 6, pp 1997–2001 | Cite as

Polymersomes, smaller than you think: ferrocene as a TEM probe to determine core structure

Brief Communication


By incorporating ferrocene into the hydrophobic membrane of PEG-b-PCL polymersome nanoparticles it is possible to selectively visualize their core using Transmission Electron Microscopy (TEM). Two different sizes of ferrocene-loaded polymersomes with mean hydrodynamic diameters of approximately 40 and 90 nm were prepared. Image analysis of TEM pictures of these polymersomes found that the mean diameter of the core was 4–5 times smaller than the mean hydrodynamic diameter. The values obtained also allow the surface diameter and internal volume of the core to be calculated.


Nanoparticle Characterization Pharmacokinetics Polymersomes PEG PCL TEM Biomedicine 


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.School of Biological SciencesUniversity of SouthamptonSouthamptonUK
  2. 2.Institute of Health and Biomedical InnovationQueensland University of TechnologyKelvin GroveAustralia
  3. 3.School of Medicine, Clinical NeurosciencesUniversity of SouthamptonSouthamptonUK

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