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Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity

Journal of Nanoparticle Research volume 13, pages 2981–2988 (2011)Cite this article

Abstract

Development of green nanotechnology is generating interest of researchers toward ecofriendly biosynthesis of nanoparticles. In this study, biosynthesis of stable silver nanoparticles was done using Tulsi (Ocimum sanctum) leaf extract. These biosynthesized nanoparticles were characterized with the help of UV–vis spectrophotometer, Atomic Absorption Spectroscopy (AAS), Dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Transmission electron microscopy (TEM). Stability of bioreduced silver nanoparticles was analyzed using UV–vis absorption spectra, and their antimicrobial activity was screened against both gram-negative and gram-positive microorganisms. It was observed that O. sanctum leaf extract can reduce silver ions into silver nanoparticles within 8 min of reaction time. Thus, this method can be used for rapid and ecofriendly biosynthesis of stable silver nanoparticles of size range 4–30 nm possessing antimicrobial activity suggesting their possible application in medical industry.

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Acknowledgments

We heartly acknowledge Dr. A. K. Chauhan for their support and providing facilities for the fulfillment of this study. We also acknowledge Dr. Richa Krishna for her guidance.

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Authors and Affiliations

  1. Amity Institute of Nanotechnology, Amity University Uttar Pradesh, Noida, Uttar Pradesh, India

    Garima Singhal, Riju Bhavesh, Kunal Kasariya & Rajendra Pal Singh

  2. Infectious Disease Medical Research Center, College of Medicine, Hallym University, Chuncheon, Gangwan Do, South Korea

    Ashish Ranjan Sharma

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  1. Garima Singhal
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  2. Riju Bhavesh
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  3. Kunal Kasariya
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  4. Ashish Ranjan Sharma
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  5. Rajendra Pal Singh
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Correspondence to Garima Singhal.

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Singhal, G., Bhavesh, R., Kasariya, K. et al. Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. J Nanopart Res 13, 2981–2988 (2011). https://doi.org/10.1007/s11051-010-0193-y

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  • DOI: https://doi.org/10.1007/s11051-010-0193-y

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