Journal of Nanoparticle Research

, Volume 13, Issue 1, pp 45–51 | Cite as

Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications

  • Gaowu W. Qin
  • Farzana Darain
  • Hui Wang
  • Krassen Dimitrov
Brief communication


We report a simple and novel method for surface biofunctionalization onto recently reported Ni80Fe20 permalloy nanoparticles (~71 nm) and the immobilization of a model protein, IgG from human serum. The strategy of protein immobilization involved attachment of histidine-tagged streptavidin to the Ni80Fe20 nanoparticles via a non-covalent ligand binding followed by biotinylated human IgG binding on the nanoparticle surface using the specific high affinity avidin–biotin interaction. The biofunctionalization of Ni80Fe20 permalloy nanoparticles was confirmed by Fourier Transform InfraRed (FTIR) spectroscopy and protein denaturing gel electrophoresis (lithium dodecyl sulfate-polyacrylamide gel electrophoresis, LDS-PAGE). This protocol for surface functionalization of the novel nanometer-sized Ni80Fe20 permalloy particles with biological molecules could open diverse applications in disease diagnostics and drug delivery.


Ni80Fe20 permalloy Magnetic nanoparticles Protein immobilization Avidin–biotin bridge Gel electrophoresis Nanomedicine 

Supplementary material

11051_2010_101_MOESM1_ESM.doc (478 kb)
Supplementary material 1 (DOC 478 kb)


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Gaowu W. Qin
    • 1
  • Farzana Darain
    • 2
  • Hui Wang
    • 2
  • Krassen Dimitrov
    • 2
  1. 1.Key Laboratory for Anisotropy and Texture of Materials (MOE)Northeastern UniversityShenyangChina
  2. 2.Australian Institute for Bioengineering and NanotechnologyThe University of QueenslandSt LuciaAustralia

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