Continuous optical coherence tomography monitoring of nanoparticles accumulation in biological tissues
- 234 Downloads
In this study, dynamics of nanoparticles penetrating and accumulating in biotissue (healthy skin) was investigated in vivo by the noninvasive method of optical coherence tomography (OCT). Gold nanoshells and titanium dioxide nanoparticles were studied. The processes of the nanoparticles penetration and accumulation in biotissue are accompanied by the changes in optical properties of skin which affect the OCT images. The continuous OCT monitoring of the process of the nanoparticles penetration into skin showed that these changes appeared in 30 min after application of nanoparticles on the surface; the time of accumulation of maximal nanoparticles concentration in skin was observed in period of 1.5–3 h after application. Numerical processing of the OCT signal exhibited the increase in contrast between upper and lower parts of dermis and contrast decay of the hair follicle border during 60–150 min. The transmission electron microscopy technique confirmed accumulation of the both types of nanoparticles in biotissue. The novelty of this study is presentation of OCT ability to in vivo monitor dynamics of nanoparticles penetration and their re-distribution within living tissues.
KeywordsNanoparticles Continuous OCT monitoring Nanoparticles accumulation In vivo study Nanomedicine Health and safety
This study was supported in part by the Science and Innovations Federal Russian Agency (projects ## 02.512.11.2244, MD-3018.2009.7), RFBR grants (09-02-97072, 09-02-12215, 09-02-00539, 09-02-97040, 10-02-00744). The authors are grateful to L.B. Snopova (Nizhny Novgorod State Medical Academy) for help in performing the microscopic analysis. Also, the authors thank the Institute of Biochemistry and Physiology of Plants for providing gold–silica nanoshells and the group of companies PROMCHIM (Perm’, Russia) for providing titanium dioxide nanoparticles.
- Elder A, Vidyasagar S, DeLouise L (2009) Physicochemical factors that affect metal andmetal oxide nanoparticle passage across epithelial barriers. Wiley Interdiscip Rev Nanomed Nanobiotechnol 1(4):434–450Google Scholar
- Gelikonov VM, Gelikonov GV, Dolin LS, Kamensky VA, Sergeev AM, Shakhova NM, Gladkova ND, Zagaynova EV (2003) Optical coherence tomography: physical principles and applications. Laser Phys 13:692–702Google Scholar
- Gobin AM, Lee MH, Halas NJ, James WD, Drezek RA, West JL (2007) Near-infrared resonant nanoshells for combined optical imaging and photothermal cancer. NanoLetters 7:1929–1934Google Scholar
- Innes B, Tsuzuki T, Dawkins H, Dunlop J, Trotter G, Nearn MR, McCormick PG, Edlich F (2002) Nanotechnology and the cosmetic chemist. Cosmet Aerosols Toilet Aust 15:21–24Google Scholar
- Kirillin M, Shirmanova M, Sirotkina M, Bugrova M, Khlebtsov B, Zagaynova E (2009) Contrasting properties of gold nanoshells and titanium dioxide nanoparticles for OCT imaging of skin: Monte Carlo simulations and in vivo study. J Biomed Opt 14:021017-1–021017-11Google Scholar
- Lademann J, Weighmann HJ, Schaefer H, Muller G, Sterry W (2000) Investigation of the stability of coated titanium microparticles in a sunscreen. Skin Pharmacol Appl Skin Physiol 13:258–264Google Scholar
- Parashar UK, Kesherwani V, Saxena PS, Srivastava A (2008) Role of nanomaterials in biotechnology. J Nanomater Biostruct 3:81–87Google Scholar
- Teichmann A, Jacobi U, Ossadnik M, Richter H, Koch S, Sterry W, Lademann J (2005) Differential stripping: determination of the amount of topically applied substances penetrated into the hair follicles. J Invest Dermatol 125:264–269Google Scholar
- Tkachuk LA, Vrublevsky SA, Khlebtsov BN, Melnicov AG, Khlebtsov NG, Zimnykov DA (2005) Optical properties of gold spheroidal particles and nanoshells: effect of the external dielectric medium. Proc SPIE Int Soc Opt Eng 5772:1–10Google Scholar
- Zagaynova EV, Shirmanova MV, Orlova AG, Balalaeva IV, Kirillin MYu, Kamensky VA, Bugrova ML, Sirotkina MA (2008a) Gold nanoshells for OCT imaging contrast: from model to in vivo study. In: Proceedings of SPIE 6865 KGoogle Scholar
- Zagaynova EV, Shirmanova MV, MYu Kirillin, Khlebtsov BN, Orlova AG, Balalaeva IV, Sirotkina MA, Bugrova ML, Agrba PD, Kamensky VA (2008b) Contrasting properties of gold nanoparticles for optical coherence tomography: phantom, in vivo studies and Monte Carlo simulation. Phys Med Biol 53:4995–5009CrossRefGoogle Scholar