Facile method for CLSM imaging unfunctionalized Au nanoparticles through fluorescent channels
- 149 Downloads
The microscopic visualization of metal nanoparticles has become a useful tool for the investigation of their applications in cell labeling and the study of their bio-effects. In the current study, we have developed a facile method with confocal laser scanning microscope (CLSM) to observe unfunctionalized Au nanoparticles through fluorescent channels. The sharp reflected signal and photostable property of the metal nanoparticles makes the present method very ideal for fluorescent co-localization, real-time imaging, and further quantitative analysis.
KeywordsAu nanoparticles CLSM Real-time imaging Instrumentation Nanomedicine
- Inoue K, Kurosumi K, Deng ZP (1982) An improvement of the device for rapid freezing by use of liquid propane and the application of immunocytochemistry to the resin section of rapid-frozen, substitution-fixed anterior pituitary gland. J Electron Microsc (Tokyo) 31:93–97Google Scholar
- Itoh J, Sanno N, Matsuno A, Itoh Y, Watanabe K, Osamura RY (1997) Application of confocal laser scanning microscopy (CLSM) to visualize prolactin (PRL) and PRL mRNA in the normal and estrogen-treated rat pituitary glands using non-fluorescent Probes. Microsc Res Tech 39:157–167. doi:10.1002/(SICI)1097-0029(19971015)39:2<157::AID-JEMT7>3.0.CO;2-I PubMedCrossRefGoogle Scholar
- Kienberger F, Stroh C, Kada G, Moser R, Baumgartner W, Pastushenko V, Rankl C, Schmidt U, Muller H, Orlova E, LeGrimellec C, Drenckhahn D, Blaas D, Hinterdorfer P (2003) Dynamic force microscopy imaging of native membranes. Ultramicroscopy 97:229–237. doi:10.1016/S0304-3991(03)00047-0 PubMedCrossRefGoogle Scholar
- Tkachenko AG, Xie H, Liu YL, Coleman D, Ryan J, Glomm WR, Shipton MK, Franzen S, Feldheim DL (2004) Cellular trajectories of peptide-modified gold particle complexes: comparison of nuclear localization signals and peptide transduction domains. Bioconjug Chem 15:482–490. doi:10.1021/bc034189q PubMedCrossRefGoogle Scholar