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Facile method for CLSM imaging unfunctionalized Au nanoparticles through fluorescent channels

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Abstract

The microscopic visualization of metal nanoparticles has become a useful tool for the investigation of their applications in cell labeling and the study of their bio-effects. In the current study, we have developed a facile method with confocal laser scanning microscope (CLSM) to observe unfunctionalized Au nanoparticles through fluorescent channels. The sharp reflected signal and photostable property of the metal nanoparticles makes the present method very ideal for fluorescent co-localization, real-time imaging, and further quantitative analysis.

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Acknowledgment

We thank the State Key Program of Basic Research (973) (2007CB808000) for financial support and Dr. Gang Hu for editing the manuscript.

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Authors and Affiliations

  1. Medical and Healthy Analytical Center, Peking University Health Science Center, 100083, Beijing, People’s Republic of China

    Lan Yuan

  2. Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, People’s Republic of China

    Wei Wei

  3. School of Public Health, Jilin University, 130021, Changchun, People’s Republic of China

    Juan Li, Zhiwei Sun, Hongfang Wang, Xiuzhi Zhang & Yueyue Chen

Authors
  1. Lan Yuan
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  2. Wei Wei
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  3. Juan Li
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  4. Zhiwei Sun
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  5. Hongfang Wang
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  6. Xiuzhi Zhang
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  7. Yueyue Chen
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Corresponding author

Correspondence to Juan Li.

Additional information

Lan Yuan and Wei Wei contributed equally to this study.

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Yuan, L., Wei, W., Li, J. et al. Facile method for CLSM imaging unfunctionalized Au nanoparticles through fluorescent channels. J Nanopart Res 11, 1219–1225 (2009). https://doi.org/10.1007/s11051-009-9618-x

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  • DOI: https://doi.org/10.1007/s11051-009-9618-x

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