A novel magnetic fluid based on starch-coated magnetite nanoparticles functionalized with homing peptide
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Preparation and characterization in vitro and in vivo of a novel magnetic fluid based on starch-coated magnetite nanoparticles functionalized with homing peptide is reported in this paper. Precursory magnetic fluids stabilized with starch were prepared, in a polymeric starch matrix, by controlled chemical coprecipitation of magnetite phase from aqueous solutions. The average hydrodynamic diameter of starch-coated iron oxide nanoparticles (SIONs) was 46 nm. As a homing peptide, A54 is the most effective peptide specific to the human hepatocellular carcinoma cell line BEL-7402. Final magnetic fluids were obtained through chemical coupling of homing peptide labeled with 5-carboxyl-fluorescein (FAM-A54) and SIONs. Magnetic measurements showed the saturation magnetization value of SIONs amounted to 45 emu/g and the FAM-A54-coupled SIONs showed a good magnetic response in magnetic field. The results of experiments in vitro and in vivo showed that SIONs were endowed with specific affinity to corresponding tumor cells after coupling with FAM-A54 and the FAM-A54-coupled SIONs could be accumulated in the tumor tissue with more efficiency than individual magnetic targeting or biomolecular targeting. This novel magnetic fluid with dual function has great potential applications in diagnostics and therapeutics of human tumor such as drug targeting, magnetic hyperthermia, and magnetic resonance imaging.
KeywordsMagnetic fluid Dual function Homing peptide Nanoparticles coupling Nanomedicine
- Bulte JWM, Zhang SC, van Gelderen P, Herynek V, Jordan EK, Duncan ID, Frank JA (1999) Neurotransplantation of magnetically labeled oligodendrocyte progenitors: MR tracking migration and myelination. Proc Natl Acad Sci USA 96(26):15256–15261. doi:10.1073/pnas.96.26.15256 PubMedCrossRefADSGoogle Scholar
- Gan ZF, Jiang JS (2005) Preparation of magnetic monodisperse nanoparticles and biopolymer assemble on the magnetic carriers. Prog Chem 17(6):978–986Google Scholar
- Mohapatra S, Mallick SK, Maiti TK, Ghosh SK, Pramanik P (2007) Synthesis of highly stable folic acid conjugated magnetite nanoparticles for targeting cancer cells. Nanotechnology 18:1–9Google Scholar
- Qian M, Zhou ZL, Zhang P, Du B, Yu J, Yu M (2004) A hepatocarcinoma-binding peptide from a phage-display random peptide library identified by in vivo panning and its application. Chinese Patent No. 1563077Google Scholar