Skip to main content
SpringerLink
Log in

Synthesis of Ni–Zn ferrite nanoparticles in radiofrequency thermal plasma reactor and their use for purification of histidine-tagged proteins

  • Technology and Applications
  • Published:
Journal of Nanoparticle Research Aims and scope Submit manuscript

Abstract

Superparamagnetic Ni–Zn ferrite nanoparticles were synthesized in radiofrequency thermal plasma reactor from aqueous solutions of Ni- and Zn-nitrates. The nanoparticles were studied for protein purification performance in both quantitative and qualitative terms. For comparison, experiments were also performed by Ni-charged affinity chromatography. It was proved that the Ni–Zn ferrite nanoparticles effectively purified histidine-tagged proteins with a maximum protein binding capacity of about 7% (w/w). Gel electrophoresis demonstrated better purification characteristics for magnetic nanoparticles than for affinity chromatography.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

RF:

Radiofrequency

SDS-PAGE:

SDS polyacrylamide gel electrophoresis

References

  • Betz N (2004) Purifying His-tagged proteins from insect and mammalian cells. Cell Notes 9:6–9

    Google Scholar 

  • Cullity BD (1972) Introduction to magnetic materials. Addison-Werley, Reading

    Google Scholar 

  • Frenzel A, Bergemann C, Köhl G, Reinard T (2003) Novel purification system for 6×His-tagged proteins by magnetic affinity separation. J Chromatogr B 793:325–329

    Article  CAS  Google Scholar 

  • Hofmann I, Schnolzer M, Kaufmann I, Franke WW (2002) Symplekin, a constitutive protein of karyo- and cytoplasmic particles involved in mRNA biogenesis in Xenopus laevis oocytes. Mol Biol Cell 13:1665–1676

    Article  CAS  Google Scholar 

  • Lim YT, Lee KY, Lee K, Chung BH (2006) Immobilization of histidine-tagged proteins by magnetic nanoparticles encapsulated with nitrilotriacetic acid (NTA)-phospholipids micelle. Biochem Biophys Res Commun 344:926–930

    Article  CAS  Google Scholar 

  • Lin C-T, Moore PA, Auberry DL, Landorf EV, Peppler T, Victry KD, Collart FR, Kery V (2006) Automated purification of recombinant proteins: combining high-throughput with high yield. Protein Expr Purif 47:16–24

    Article  CAS  Google Scholar 

  • Minamino T, Macnab RM (2000) Interactions among components of the Salmonella flagellar export apparatus and its substrates. Mol Microbiol 35(5):1052–1064

    Article  CAS  Google Scholar 

  • Porath J (1992) Immobilized metal ion affinity chromatography. Protein Expr Purif 3:263–281

    Article  CAS  Google Scholar 

  • Safarik I, Safarikova M (2004) Magnetic techniques for the isolation and purification of proteins and peptides. Biomagn Res Technol 2(7)

  • Scheich C, Sievert V, Büssow K (2003) An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography. BMC Biotechnol 3(12)

  • Szépvölgyi J, Gál L, Mohai I, Mészáros I, Gubicza J (2007) Synthesis of spinel ferrites in RF thermal plasma reactor. Paper no. 30P-150, Proc. ISPC 18, Kyoto, Japan, ISBN 978-4-9903773-3-5

  • Tartaj P, Morales MP, Gonzalez-Carreno T, Veintemillas-Verdaguer S, Serna CJ (2005) Advances in magnetic nanoparticles for biotechnology applications. J Magn Magn Mater 290–291:28–34

    Article  Google Scholar 

  • van den Berg S, Löfdahl PA, Härd T, Berglund H (2006) Improved solubility of TEV protease by directed evolution. J Biotechnol 121:291–298

    Article  Google Scholar 

Download references

Acknowledgment

This work has been supported by the Hungarian Scientific Research Fund Grant No. T047360.

Author information

Authors and Affiliations

  1. Institute of Materials and Environmental Chemistry, Chemical Research Center, Hungarian Academy of Sciences, Pusztaszeri u. 59-67, 1025, Budapest, Hungary

    Tivadar Feczkó, Loránd Gál & János Szépvölgyi

  2. Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Egyetem u. 2, 8200, Veszprem, Hungary

    Tivadar Feczkó & János Szépvölgyi

  3. Department of Nanotechnology, Faculty of Information Technology, University of Pannonia, Egyetem u. 10, 8200, Veszprem, Hungary

    Adél Muskotál & Ferenc Vonderviszt

  4. Research Institute for Technical Physics and Materials Science, Hungarian Academy of Sciences, Konkoly-Thege Miklós út 29-33, 1121, Budapest, Hungary

    Anett Sebestyén & Ferenc Vonderviszt

  5. Institute of Enzymology, Hungarian Academy of Sciences, Karolina út 29, 1113, Budapest, Hungary

    Ferenc Vonderviszt

Authors
  1. Tivadar Feczkó
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Adél Muskotál
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Loránd Gál
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. János Szépvölgyi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Anett Sebestyén
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ferenc Vonderviszt
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tivadar Feczkó.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Feczkó, T., Muskotál, A., Gál, L. et al. Synthesis of Ni–Zn ferrite nanoparticles in radiofrequency thermal plasma reactor and their use for purification of histidine-tagged proteins. J Nanopart Res 10 (Suppl 1), 227–232 (2008). https://doi.org/10.1007/s11051-008-9429-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11051-008-9429-5

Keywords

Search

Navigation