Journal of Nanoparticle Research

, Volume 11, Issue 4, pp 775–783 | Cite as

Aptamer-modified gold nanoparticles for targeting breast cancer cells through light scattering

  • Yu-Fen Huang
  • Yang-Wei Lin
  • Zong-Hong Lin
  • Huan-Tsung Chang
Research Paper

Abstract

In this study, we demonstrated the potential use of nucleic acid ligand (aptamers) conjugated gold nanoparticles (AuNPs) for cancer cell detection. Through specific binding of the aptamers toward platelet-derived growth factor (PDGF), MDA-MB-231 and Hs578T cells (cancer cells) that over-express PDGF, interact with Apt-AuNPs to a greater extent than do H184B5F5/M10 cells (normal cells). These results were confirmed through inductively coupled plasma mass spectrometry measurements of the gold ion concentrations within these cells. Aggregation of the Apt-AuNPs in the cytoplasm of the cancer cells led to the generation of an intense scattered light upon photo-illumination; this phenomenon allows the differentiation of cancer cells from normal cells using a dark field optical microscope. The presence of Apt-AuNPs suppressed the proliferation of MDA-MB-231 cancer cells, but not H184B5F5/M10 cells.

Keywords

Aptamer Gold nanoparticles Breast cancer cells Imaging Proliferation Nanomedicine 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Yu-Fen Huang
    • 1
  • Yang-Wei Lin
    • 1
  • Zong-Hong Lin
    • 1
  • Huan-Tsung Chang
    • 1
    • 2
  1. 1.Department of ChemistryNational Taiwan UniversityTaipeiTaiwan, ROC
  2. 2.Department of Natural Science EducationNational Taitung UniversityTaitungTaiwan, ROC

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