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Candida massiliensis sp. nov. Isolated from a Clinical Sample

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Abstract

The majority of Candida species are known as non-pathogenic yeasts and rarely involved in human diseases. However, recently case reports of human infections caused by non-albicans Candida species have increased, mostly in immunocompromised hosts. Our study aimed to describe and characterize as thoroughly as possible, a new species of the Metschnikowia clade, named here Candida massiliensis (PMML0037), isolated from a clinical sample of human sputum. We targeted four discriminant genetic regions: “Internal Transcribed Spacers” of rRNA, D1/D2 domains (28S large subunit rRNA) and part of the genes encoding Translation Elongation Factor 1-α and β-tubulin2. The genetic data were compared to morphological characters, from scanning electron microscopy (TM 4000 Plus, SU5000), physiological, including the results of oxidation and assimilation tests of different carbon sources by the Biolog system, and chemical mapping by Energy-Dispersive X-ray Spectroscopy. Lastly, the in vitro antifungal susceptibility profile was performed using the E-test™ exponential gradient method. The multilocus analysis supported the genetic position of Candida massiliensis (PMML0037) as a new species of the Metschnikowia clade, and the phenotypic analysis highlighted its unique morphological and chemical profile when compared to the other Candida/Metschnikowia species included in the study.

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Data Availability

The Candida massiliensis holotype is available in the IHU MI (no. PMML0037) and IHEM (no. 28560) strain collections. The nucleotide sequences are available in GenBank (accession nos. OM952170 and OM952176). The datasets analysed in the current study are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors gratefully acknowledge wholehearted and fruitful discussions with Pierre Pontarotti concerning phylogenetic analysis and the technical support of Anthony Fontanini for electron microscopy investigations. We also sincerely thank Takashi Irie, Kyoko Imai, Shigeki Matsubara, Taku Sakazume, Toshihide Agemura, and the Hitachi team in Japan for the collaborative study conducted together with IHU Méditerranée Infection and for the installation of TM4000 Plus microscopes at the IHU Méditerranée Infection facility.

Funding

This research was funded by the Institut Hospitalo-Universitaire (IHU) Méditerranée Infection and by the French government under the “Investissements d’avenir” (Investments for the Future) programme managed by the Agence Nationale de la Recherche (ANR, fr: National Agency for Research), (reference: Méditerranée Infection 10-IAHU-03) and the Région Provence-Alpes-Côte d’Azur, European ERDF funding (European Regional Development Fund) and PRIMMI (Plateformes de Recherche et d’Innovation Mutualisées Méditerranée Infection).

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Authors and Affiliations

  1. IHU Méditerranée Infection, 13005, Marseille, France

    Jihane Kabtani, Fatima Boulanouar, Muriel Militello, Carole Cassagne & Stéphane Ranque

  2. AP-HM, IRD, SSA, MEPHI, Aix-Marseille Université, 13005, Marseille, France

    Muriel Militello

  3. AP-HM, IRD, SSA, VITROME, Aix-Marseille Université, 13005, Marseille, France

    Carole Cassagne & Stéphane Ranque

Authors
  1. Jihane Kabtani
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  2. Fatima Boulanouar
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  3. Muriel Militello
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  4. Carole Cassagne
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  5. Stéphane Ranque
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by JK, FB, and MM. The first draft of the manuscript was written by JK and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Stéphane Ranque.

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Kabtani, J., Boulanouar, F., Militello, M. et al. Candida massiliensis sp. nov. Isolated from a Clinical Sample. Mycopathologia 188, 957–971 (2023). https://doi.org/10.1007/s11046-023-00792-4

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