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Occurrence of Cystic Fibrosis Transmembrane Conductance Regulator Gene Mutations in Patients with Allergic Bronchopulmonary Aspergillosis Complicating Asthma

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Abstract

Background

Whether cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations contribute to the high prevalence of allergic bronchopulmonary aspergillosis (ABPA) in India remains unknown. We aimed to evaluate the occurrence of CFTR mutations in subjects with ABPA complicating asthma.

Methods

We sequenced the CFTR gene using genomic DNA from blood on the Illumina NextSeq500 platform. Before undertaking zygosity analysis by genome analysis toolkit, the known or novel single nucleotide polymorphisms (SNPs) and indels were called. For rigorous analysis, we included only high-quality SNPs (scores > 500) and coverage ranging from 30 × 150x.

Results

We included 18, 12, and eight adult participants of ABPA, asthma, and healthy controls, respectively. The frequency of SNPs was higher in asthmatic subjects than ABPA or healthy controls, albeit not statistically significant (9/12 [75%] vs. 11/18 [61.1%] vs. 3/8 [37.5%], p = 0.24). Of the 38 subjects, 23 yielded 50 variants (healthy controls [n = 5], ABPA [n = 22], asthma [n = 23]) corresponding to six SNPs not previously linked with ABPA. Of these, four SNPs (rs213950, rs200735475, rs1800113, and rs1800136) were catalogued in the NCBI database. We identified two novel SNPs (chr7:117250703, chr7:117282655) in four (ABPA [n = 1], asthma [n = 3]) subjects without corresponding reference SNP. Most SNPs (85.5%) were heterozygous. The frequency of SNPs was higher in ABPA subjects with high-attenuation mucus (52.2%) and bronchiectasis (39.1%) than serological ABPA (8.7%).

Conclusions

Our study suggests the role of CFTR mutations in the pathogenesis of ABPA. The SNPs in the CFTR gene may contribute to disease severity in ABPA. Larger studies are required to confirm our findings.

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Data availability statement

Data will be made available on request with the corresponding author.

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Funding

We acknowledge the partial financial support provided by the International Society for Human and Animal Mycology (ISHAM) through the ISHAM-ABPA working group.

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Authors and Affiliations

  1. Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research (PGIMER), Sector-12, Chandigarh, 160012, India

    Rimjhim Kanaujia, Amit Arora, Arunaloke Chakrabarti & Shivaprakash M. Rudramurthy

  2. Department of Pulmonary Medicine, Post Graduate Institute of Medical Education and Research (PGIMER), Sector-12, Chandigarh, 160012, India

    Ritesh Agarwal

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  1. Rimjhim Kanaujia
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Contributions

RK: data collection, preparation of the first draft, and revision of the manuscript, AA: data collection, data analysis, preparation of the first draft, and revision of the manuscript, AC: data collection and revision of the manuscript, SMR: data collection and revision of the manuscript, RA: conceptualization of the project, data collection, preparation of the first draft, and revision of the manuscript.

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Correspondence to Amit Arora or Ritesh Agarwal.

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This is an observational study. The Institute Ethics Committee approved the study protocol.

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We obtained written informed consent from all the study subjects. The procedures used in this study adhere to the tenets of the Declaration of Helsinki.

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Kanaujia, R., Arora, A., Chakrabarti, A. et al. Occurrence of Cystic Fibrosis Transmembrane Conductance Regulator Gene Mutations in Patients with Allergic Bronchopulmonary Aspergillosis Complicating Asthma. Mycopathologia 187, 147–155 (2022). https://doi.org/10.1007/s11046-022-00631-y

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