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Complete Genome Sequence of Candida theae from Hickman Line Infection in an Immunocompromised Child


We present the first whole-genome-sequencing data of a rare fungal species, Candida theae isolated in the context of a Hickman line infection in a patient with juvenile myelomonocytic leukaemia. To our knowledge, this is the first instance of publicly available genomic data for this species. Loci associated with antifungal resistance were referenced against its closely related members of the Candida parapsilosis complex.

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  1. Chang C-F, Lin Y-C, Chen S-F, et al. Candida theae sp. nov., a new anamorphic beverage-associated member of the Lodderomyces clade. Int J Food Microbiol. 2012;153:10–4.

    Article  PubMed  Google Scholar 

  2. Yang Z, Wu L, Liu X, et al. Epidemiology, species distribution and outcome of nosocomial Candida spp. bloodstream infection in Shanghai. BMC Infect Dis. 2014;14:241.

    Article  PubMed  PubMed Central  Google Scholar 

  3. White TJ, Bruns S, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protoc Guid Methods Appl. 1990;18:315–22.

    Google Scholar 

  4. Koren S, Walenz BP, Berlin K, et al. Canu: scalable and accurate long-read assembly via adaptive κ-mer weighting and repeat separation. Genome Res. 2017.

    Article  PubMed  PubMed Central  Google Scholar 

  5. Walker BJ, Abeel T, Shea T, et al. Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement. PLoS ONE. 2014.

    Article  PubMed  PubMed Central  Google Scholar 

  6. Chakraborty M, Baldwin-Brown JG, Long AD, Emerson JJ. Contiguous and accurate de novo assembly of metazoan genomes with modest long read coverage. Nucleic Acids Res. 2016;44:e147.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  7. Mikheenko A, Prjibelski A, Saveliev V, et al. Versatile genome assembly evaluation with QUAST-LG. Bioinformatics. 2018;34:i142–50.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  8. Richter M, Rossello-Mora R, Oliver Glockner F, Peplies J. JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics. 2016;32:929–31.

    Article  PubMed  CAS  Google Scholar 

  9. The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs for antifungal agents, version 10.0, 2020. The European Committee on Antimic 2020. No Title.

  10. Martí-Carrizosa M, Sánchez-Reus F, March F, et al. Implication of Candida parapsilosis FKS1 and FKS2 mutations in reducing echinocandin susceptibility. Antimicrob Agents Chemother. 2015;59:3570–3.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  11. Edlind TD, Katiyar SK. Mutational analysis of flucytosine resistance in Candida glabrata. Antimicrob Agents Chemother. 2010.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Mac Aogáin M, Chaturvedi V, Chotirmall SH. MycopathologiaGENOMES: the New “Home” for the Publication of Fungal Genomes. Mycopathologia. 2019;184:551–4.

    Article  Google Scholar 

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This research is supported by the NUHS Internal Grant Funding under the NUHS Seed Fund (NUHSRO/2018/082/RO5+5/Seed-Aug/08).

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KLC, SO, JT conceived and planned the experiments. SO and JT performed genomic analysis. KLC contributed clinical input. KLC took the lead in writing the manuscript. All authors provided critical feedback and helped shape the research, analysis and manuscript.

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Correspondence to Jeanette W. P. Teo.

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The authors have no conflict of interest to declare.

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Chew, K.L., Octavia, S. & Teo, J.W.P. Complete Genome Sequence of Candida theae from Hickman Line Infection in an Immunocompromised Child. Mycopathologia 185, 727–729 (2020).

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  • Whole-genome-sequencing
  • Candida theae
  • Antifungal susceptibility
  • 5-Flucytosine resistance
  • Antimicrobial resistance