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Immunodiagnostic Assays for the Investigation of Fungal Outbreaks

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Abstract

Fungal pathogens can affect humans, animals, and plants, and they can be found in the environment or as part of the host microbiome. Fungal diseases present a broad clinical spectrum, ranging from superficial to invasive infections, and can cause outbreaks. During an outbreak investigation, the laboratory plays an essential role in verifying the diagnosis and helping to confirm the source of the outbreak. Immunodiagnostic assays are important tools and often relied upon for the diagnosis of fungal infections, since the gold standard assays of culture and histopathology are time-consuming and often require invasive procedures. Immunodiagnostic assays range from complement fixation and immunodiffusion to enzyme immunoassays and, most recently, to point-of-care lateral flow devices. In general, these assays provide results faster and offer good analytical performance. These characteristics make immunodiagnostic assays good laboratory tools for outbreak investigations. The aim of this review is to describe the principles, advantages, limitations, and availability of immunodiagnostics assays in outbreak investigations, based on the experience of a reference laboratory.

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Authors and Affiliations

  1. Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, 30333, USA

    Diego H. Caceres, Tom Chiller & Mark D. Lindsley

  2. Center of Expertise in Mycology Radboudumc/CWZ, Nijmegen, The Netherlands

    Diego H. Caceres

  3. Mycotic Diseases Branch, Centers for Disease Control and Prevention (CDC), 1600 Clifton Rd. NE, MS H17-2, Atlanta, GA, 30329-4027, USA

    Mark D. Lindsley

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Caceres, D.H., Chiller, T. & Lindsley, M.D. Immunodiagnostic Assays for the Investigation of Fungal Outbreaks. Mycopathologia 185, 867–880 (2020). https://doi.org/10.1007/s11046-020-00452-x

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