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Candida albicans Elicits Pro-Inflammatory Differential Gene Expression in Intestinal Peyer’s Patches

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Abstract

The details of how gut-associated lymphoid tissues such as Peyer’s patches (PPs) in the small intestine play a role in immune surveillance, microbial differentiation and the mucosal barrier protection in response to fungal organisms such as Candida albicans are still unclear. We particularly focus on PPs as they are the immune sensors and inductive sites of the gut that influence inflammation and tolerance. We have previously demonstrated that CD11c+ phagocytes that include dendritic cells and macrophages are located in the sub-epithelial dome within PPs sample C. albicans. To gain insight on how specific cells within PPs sense and respond to the sampling of fungi, we gavaged naïve mice with C. albicans strains ATCC 18804 and SC5314 as well as Saccharomyces cerevisiae. We measured the differential gene expression of sorted CD45+ B220+ B-cells, CD3+ T-cells and CD11c+ DCs within the first 24 h post-gavage using nanostring nCounter® technology. The results reveal that at 24 h, PP phagocytes were the cell type that displayed differential gene expression. These phagocytes were able to sample C. albicans and discriminate between strains. In particular, strain ATCC 18804 upregulated fungal-specific pro-inflammatory genes pertaining to innate and adaptive immune responses. Interestingly, PP CD11c+ phagocytes also differentially expressed genes in response to C. albicans that were important in the protection of the mucosal barrier. These results highlight that the mucosal barrier not only responds to C. albicans, but also aids in the protection of the host.

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Acknowledgements

We thank the Wadsworth Center Applied Genomic Technologies Core and the Wadsworth Center Biochemistry and Immunology Core. We thank Dr. Kelly Miller of Nanostring Technologies for assistance with technical support and data analysis. We thank Yannick David for his help in generating the PP model. We thank Dr. Sudha Chaturvedi at the Wadsworth Center Mycology laboratory for providing C. albicans strain ATCC 18804. We thank Dr. Aaron Mitchell at Carnegie Mellon University for providing C. albicans strain SC5314. We thank Dr. Kimberly McClive-Reed of Health Research, Inc. for critical reading of this manuscript. M.D.J. was supported by the University at Albany and Wadsworth Center start-up funds. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

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  1. Navjot Singh & Heather C. Kim

    Present address: , 120 New Scotland Avenue, Albany, NY, 12208, USA

  2. Navjot Singh and Heather C. Kim have contributed equally to this work.

Authors and Affiliations

  1. Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, NY, USA

    Heather C. Kim, Steven R. Torres & Magdia De Jesus

  2. Division of Infectious Diseases, New York State Department of Health, Wadsworth Center, Albany, NY, USA

    Heather C. Kim, Jaskiran K. Dhinsa, Steven R. Torres & Magdia De Jesus

  3. Applied Genomics Technology Core, New York State Department of Health, Wadsworth Center, Albany, NY, USA

    Navjot Singh

  4. Biochemistry and Immunology Core, New York State Department of Health, Wadsworth Center, Albany, NY, USA

    Renjie Song

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Singh, N., Kim, H.C., Song, R. et al. Candida albicans Elicits Pro-Inflammatory Differential Gene Expression in Intestinal Peyer’s Patches. Mycopathologia 184, 461–478 (2019). https://doi.org/10.1007/s11046-019-00349-4

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