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Corneal Collagen Cross-Linking for the Management of Mycotic Keratitis

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To evaluate the efficiency of corneal collagen cross-linking (CXL) in addition to topical voriconazole in cases with mycotic keratitis.


Retrospective case series in a tertiary university hospital.


CXL was performed on 13 patients with mycotic keratitis who presented poor or no response to topical voriconazole treatment.


The clinical features, symptoms, treatment results and complications were recorded retrospectively. The corneal infection was graded according to the depth of infection into the stroma (from grade 1 to grade 3). The visual analogue scale was used to calculate the pain score before and 2 days after surgery.

Main Outcome Measures

Grade of the corneal infection.


Mean age of 13 patients (6 female and 7 male) was 42.4 ± 17.7 years (20–74 years). Fungus was demonstrated in culture (eight patients) or cytological examination (five patients). Seven of the 13 patients (54%) were healed with topical voriconazole and CXL adjuvant treatment in 26 ± 10 days (15–40 days). The remaining six patients did not respond to CXL treatment; they initially presented with higher grade ulcers. Pre- and post-operative pain score values were 8 ± 0.8 and 3.5 ± 1, respectively (p < 0.05).


The current study suggests that adjunctive CXL treatment is effective in patients with small and superficial mycotic ulcers. These observations require further research by large randomized clinical trials.

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Corneal collagen cross-linking


Sabouraud glucose agar


Potato dextrose agar


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Correspondence to Ibrahim Inan Harbiyeli.

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Erdem, E., Harbiyeli, I.I., Boral, H. et al. Corneal Collagen Cross-Linking for the Management of Mycotic Keratitis. Mycopathologia 183, 521–527 (2018).

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