Sporotrichosis is a chronic infection caused by the dimorphic fungus Sporothrix schenckii, involving all layers of skin and the subcutaneous tissue. The role of innate immune toll-like receptors 2 and 4 in the defense against this fungus has been reported, but so far, there were no studies on the effect of cell wall major components over the cytosolic oligo-merization domain (NOD)-like receptors, important regulators of inflammation and responsible for the maturation of IL-1β and IL-18, whose functions are dependents of the caspase-1 activation, that can participate of inflammasome. It was evaluated the percentage of activation of caspase-1, the production of IL-1β, IL-18, IL-17, IFN-γ and nitric oxide in a Balb/c model of S. schenckii infection. It was observed a decreased activity of caspase-1 during the fourth and sixth weeks of infection accompanied by reduced secretion of the cytokines IL-1β, IL-18 and IL-17 and high production of nitric oxide. IFN-γ levels were elevated during the entire time course of infection. This temporal reduction in caspase-1 activity coincides exactly with the reported period of fungal burden associated with a transitory immunosuppression induced by this fungus and detected in similar infection models. These results indicate the importance of interaction between caspase-1, cytokines IL-1β and IL-18 in the host defense against S. schenckii infection, suggesting a participation the inflammasome in this response.
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Brain heart infusion
Peritoneal exudate cells
Enzyme-linked immunosorbent assay
Pattern recognition receptors
Pathogen-associated molecular patterns
Protein associated with apoptosis
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The authors are grateful to Marisa Campos Polesi Placeres for technical support. This work was supported by grants from the State of Sao Paulo Research Foundation (FAPESP) (Grants No. 2013/03190-5), National Council of Scientific and Technological Development (CNPQ) and Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior (CAPES, Brazil) and Foreigner Visiting Professor Program (Grant 07610130).
The authors declare that the experiments comply with the laws in force in the country in which they were performed.
Conflict of interest
The authors declare that there is no conflict of interest.
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Gonçalves, A.C., Maia, D.C.G., Ferreira, L.S. et al. Involvement of Major Components from Sporothrix schenckii Cell Wall in the Caspase-1 Activation, Nitric Oxide and Cytokines Production During Experimental Sporotrichosis. Mycopathologia 179, 21–30 (2015). https://doi.org/10.1007/s11046-014-9810-0
- Nitric oxide
- Sporothrix schenckii