Skip to main content
SpringerLink
Log in

The MAT1-1:MAT1-2 Ratio of Sporothrix globosa Isolates in Japan

  • Published:
Mycopathologia Aims and scope Submit manuscript

Abstract

In order to understand the reproductive biology of pathogenic species in the Sporothrix schenckii complex, we characterized the partial mating type (MAT1-1) loci of Sporothrix schenckii, as well as the S. globosa MAT1-1-1 gene, which encoded 262 amino acid sequences. The data confirmed that the MAT1-1 locus of S. globosa was divergent from the MAT1-2 locus of the opposite mating type, suggesting that the fungus is heterothallic. To determine the mating type ratio of 20 isolates from Japanese patients, we analyzed the MAT loci by specific PCR amplification of MAT1-1-1 and MAT1-2-1 genes. The MAT1-1-1 was detected in 5 isolates but not in the other 15 isolates with the presence of MAT1-2-1. The MAT1-1:1-2 ratio of S. globosa isolates in Japan was estimated to be 1:3. Phylogenetic analysis indicated that the sequences of the MAT1-1-1 were identical among S. globosa isolates but different from S. schenckii and Ophiostoma montium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Berbee ML, Taylor JW. 18S Ribosomal RNA gene sequence characters place the human pathogen Sporothrix schenckii in the genus Ophiostoma. Exp Mycol. 1992;16:87–91.

    Article  CAS  Google Scholar 

  2. Bubnick M, Smulian AG. The MAT1 locus of Histoplasma capsulatum is responsive in a mating type-specific manner. Eukaryot Cell. 2007;6:616–21.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  3. Casselton LA. Fungal sex genes-searching for the ancestors. BioEssays. 2008;30:711–4.

    Article  CAS  PubMed  Google Scholar 

  4. de Beer ZW, Harrington TC, Vismer HF, Wingfield BD, Wingfield MJ. Phylogeny of the Ophiostoma stenoceras–Sporothrix schenckii complex. Mycologia. 2003;95:434–41.

    Article  PubMed  Google Scholar 

  5. de Meyer EM, de Beer ZW, Summerbell RC, Moharram AM, de Hoog GS, Vismer HF, Wingfield MJ. Taxonomy and phylogeny of new wood- and soil-inhabiting Sporothrix species in the Ophiostoma stenoceras–Sporothrix schenckii complex. Mycopatologia. 2008;100:647–61.

    Google Scholar 

  6. Hinrikson HP, Hurst SF, Lott TJ, Warnock DW, Morrison CJ. Assessment of ribosomal large-subunit D1–D2, internal transcribed spacer 1, and internal transcribed spacer 2 regions as target for molecular identification of medically important Aspergillus species. J Clin Microbiol. 2005;43:2092–103.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Kano R, Kawasaki M, Mochizuki T, Hiruma M, Hasegawa A. Mating genes of the Trichophyton mentagrophytes complex. Mycopathologia. 2012;173:103–12.

    Article  CAS  PubMed  Google Scholar 

  8. Kano R, Anzawa K, Mochizuki T, Nishimoto K, Hiruma M, Kamata H, Hasegawa A. Sporothrix schenckii (sensu strict S. globosa) mating type (MAT1-2) gene. J Dermatol. 2013;40:726–30.

    Article  CAS  PubMed  Google Scholar 

  9. Kwon-Chung KJ, Bennett JE. Distribution of α and a mating types of Cryptococcus neoformans among natural and clinical isolates. Am J Epidemiol. 1978;108:337–40.

    CAS  PubMed  Google Scholar 

  10. Kwon-Chung KJ, Bartlett MS, Wheat LJ. Distribution of the two mating types among Histoplasma capsulatum isolates obtained from an urban histoplasmosis outbreak. Sabouraudia. 1984;22:155–7.

    Article  CAS  PubMed  Google Scholar 

  11. Kwon-Chung KJ, Bennett EJ. Dermatophytoses: medical mycology. Philadelphia: Lea & Febiger; 1992. pp. 707–729 and 816–826.

  12. Marimon R, Cano J, Gené J, Sutton DA, Kawasaki M, Guarro J. Sporothrix brasiliensis, S. globosa, and S. mexicana, three new Sporothrix species of clinical interest. J Clin Microbiol. 2007;45:3198–206.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. Marimon R, Gené J, Cano J, Guarro J. Sporothrix luriei: a rare fungus from clinical origin. Med Mycol. 2008;46:621–5.

    Article  PubMed  Google Scholar 

  14. Rodrigues AM, de Hoog S, de Camargo ZP. Emergence of pathogenicity in the Sporothrix schenckii complex. Med Mycol. 51: 405–412.

  15. Rodrigues AM, de Hoog GS, Zhang Y, de Camargo ZP. Emerging sporotrichosis is driven by clonal and recombinant Sporothrix species. Emerg Microbes Infect. 2014;3:e32. doi:10.1038/emi.2014.33.

    Article  PubMed Central  Google Scholar 

  16. Romeo O, Scordino F, Criseo G. New insight into molecular phylogeny and epidemiology of Sporothrix schenckii species complex based on calmodulin-encoding gene analysis of Italian isolates. Mycopathologia. 2011;72:179–86.

    Article  Google Scholar 

  17. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28:2731–9.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994;22:4673–80.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. Tsui CK, DiGuistini S, Wang Y, Feau N, Dhillon B, Bohlmann J, Hamelin RC. Unequal recombination and evolution of the mating-type (MAT) loci in the pathogenic fungus Grosmannia clavigera and relatives. G3: Genes| Genomes| Genetics. 2013;3:465–480.

  20. Vásquez-del-Mercado E, Arenas R, Padilla-Desgarenes C. Sporotrichosis. Clin Dermatol. 2012;30:437–43.

    Article  PubMed  Google Scholar 

  21. Zhou X, Rodrigues AM, Feng P, de Hoog GS. Global ITS diversity in the Sporothrix schenckii complex. Fungal Divers. 2014;66:153–65.

    Google Scholar 

  22. Zipfel RD, de Beer ZW, Jacobs K, Wingfield BD, Wingfield MJ. Multigene phylogenies define Ceratocystiopsis and Grosmannia distinct from Ophiostoma. Stud Mycol. 2006;55:77–99.

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by grants from the Academic Frontier Project of the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) and Nihon University Multidisciplinary Research Grant for (2013).

Author information

Authors and Affiliations

  1. Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866 Kameino, Fujisawa, Kanagawa, 252-8510, Japan

    Rui Kano & Hiroshi Kamata

  2. Department of Forest and Conservation Sciences, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada

    Clement K.-M. Tsui & Richard C. Hamelin

  3. Department of Dermatology, Kanazawa Medical University, Uchinada, Ishikawa, 920-0293, Japan

    Kazushi Anzawa & Takashi Mochizuki

  4. Division of Dermatology, Ekisaikai Nagasaki Hospital, 5-16 Kabashimacho, Nagasaki-City, Nagasaki, 850-0034, Japan

    Katsutaro Nishimoto

  5. Ochanomizu Institute for Medical Mycology and Allergology, Nakamura Bldg. 2F, 2-12-4 Hongo, Bunkyoku, Tokyo, 113-0033, Japan

    Masataro Hiruma

  6. Teikyo University Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo, 192-0395, Japan

    Atsuhiko Hasegawa

Authors
  1. Rui Kano
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Clement K.-M. Tsui
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Richard C. Hamelin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kazushi Anzawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takashi Mochizuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Katsutaro Nishimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Masataro Hiruma
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hiroshi Kamata
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Atsuhiko Hasegawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rui Kano.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kano, R., Tsui, C.KM., Hamelin, R.C. et al. The MAT1-1:MAT1-2 Ratio of Sporothrix globosa Isolates in Japan. Mycopathologia 179, 81–86 (2015). https://doi.org/10.1007/s11046-014-9808-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11046-014-9808-7

Keywords

Search

Navigation