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Phylogenetic Analyses on the Diversity of Aspergillus fumigatus Sensu Lato Based on Five Orthologous Loci

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An Erratum to this article was published on 01 November 2014

Abstract

One hundred isolates of Aspergillus fumigatus sensu lato mainly from China, as well as from Australia, France, India, Indonesia, Ireland, UK, and USA were analyzed to infer their sequence types (STs) and population diversity based on partial calmodulin, calcineurin regulatory subunit B, beta-tubulin, cytochrome C and calcineurin catalytic subunit A genes as well as their mating types, using ClonalFrame, Structure and MEGA software. Our results inferred 48 STs and showed that most of the STs or lineages evolved independently and without clear population structure among them. Whereas one lineage was recognized that could be a true population and in which one clade might diverge into another distinct lineage, namely, a cryptic species, A. neoellipticus. In addition, we found that mutation, parasexual, and sexual recombination could, respectively, play specific roles in the evolution of these fungi. Our results also showed that MAT1-1/MAT1-2 mating type ratios of A. fumigatus sensu lato was biased to nearly 1:1.4 (20/28) when clone-corrected, but when not clone-corrected, the ratio of MAT1-1/MAT1-2 was so biased as near 1:2 (35/65), which might mean that isolates with MAT1-2 are in the process of losing sexual ability preceding those with MAT1-1.

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Acknowledgments

Qi-Ming Wang offered help in analyzing the data. Yun Yu and Lei Zhang did a portion of molecular work. This work is supported by National Natural Science Foundation of China (NSFC No. 31270539).

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Authors and Affiliations

  1. Department of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China

    Fang Li & Bin Cao

  2. College of Forestry, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Bo Wang

  3. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Long Wang

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  1. Fang Li
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Correspondence to Long Wang or Bin Cao.

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Li, F., Wang, B., Wang, L. et al. Phylogenetic Analyses on the Diversity of Aspergillus fumigatus Sensu Lato Based on Five Orthologous Loci. Mycopathologia 178, 163–176 (2014). https://doi.org/10.1007/s11046-014-9790-0

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