Abstract
Keratitis due to Auerswaldia lignicola in a 32-year-old Indian male carpenter is described. At presentation, the patient reported persistent pain and tearing (left eye) in spite of topical antimicrobial therapy for more than 3 weeks. Clinically, mycotic keratitis was suspected, and direct microscopy of corneal scrapings stained by lactophenol cotton blue and Gram stains revealed broad septate hyphae. Intensive topical antifungal therapy was then given for 15 days. The keratitis continued to progress, necessitating therapeutic penetrating keratoplasty. Following the keratoplasty, there was rapid reduction in inflammation and gradual quietening of the eye. Brown–black fungal colonies resembling Lasiodiplodia theobromae were isolated from corneal scrape and corneal button (post-surgery) material on Sabouraud glucose–neopeptone agar; however, sporulation did not occur, so the morphological identification could not be confirmed. Sequence analysis of the 18S rRNA region of extracted fungal genomic DNA yielded an identification of A. lignicola Ariyawansa, J.K. Liu & K.D. Hyde; the sequence data have been deposited in GenBank (A. lignicola strain DK/V4, accession number KC866317.1). Medical management of keratitis due to such rarely reported fungal species may be difficult, necessitating surgical procedures.
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References
Thomas PA. Current perspectives on ophthalmic mycoses. Clin Microbiol Rev. 2003;16:730–97.
Liu JK, Phookamsak R, Doilom M, et al. Towards a natural classification of Botryosphaeriales. Fungal Divers. 2012;57:149–210.
Kaliamurthy J, Kalavathy CM, Ramalingam DK, Prasanth DA, Jesudasan AN, Thomas PA. Keratitis due to a coelomycetous fungus: case reports and review of the literature. Cornea. 2004;23:3–12.
Kaliamurthy J, Jesudasan CAN, Prasanth DA, Thomas PA. Keratitis due to Cylindrocarpon lichenicola. J Postgrad Med. 2006;52:155–7.
Guarro J, Vieira LA, De FD, et al. Phaeoisaria clematidis as a cause of keratomycosis. J Clin Microbiol. 2000;38:2434–7.
Jani BR, Rinaldi MG, Reinhart WJ. An unusual case of fungal keratitis: Metarhizium anisopliae. Cornea. 2001;20:765–8.
Guarro J, Hofling-Lima AL, Gene J, et al. Corneal ulcer caused by the new fungal species Sarcopodium rotulorum. J Clin Microbiol. 2002;40:3071–5.
Rishi K, Font RL. Keratitis caused by an unusual fungus phoma species. Cornea. 2003;22:166–8.
Saha S, Sengupta J, Banerjee D, Khetan A, Mandal SM. Schizophyllum commune: a new organism in eye infection. Mycopathologia. 2013;175:357–60.
Karsten E, Watson SL, Foster LJ. Diversity of microbial species implicated in keratitis: a review. Open Ophthalmol J. 2012;6:110–24.
Saha S, Sengupta J, Banerjee D, Khetan A. Lasiodiplodia theobroma keratitis: a case report and review of literature. Mycopathologia. 2012;174:335–9.
Thomas PA, Teresa PA, Theodore J, Geraldine P. PCR for the molecular diagnosis of mycotic keratitis. Expert Rev Mol Diagn. 2012;12:703–18.
Rebell G, Forster RK. Lasiodiplodia theobromae as a cause of keratomycoses. Sabouraudia. 1976;14:155–70.
Borderie VM, Bourcier TM, Poirot JL, Baudrimont M, de Prudhomme Saint-Maur P, Laroche L. Endophthalmitis after Lasiodiplodia theobromae corneal abscess. Graefes Arch Clin Exp Ophthalmol. 1997;235:259–61.
Thomas PA, Garrison RG, Jansen T. Intrahyphal hyphae in corneal tissue from a case of keratitis due to Lasiodiplodia theobromae. J Med Vet Mycol. 1991;29:263–7.
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Financial assistance provided by the Bharathidasan University, Trichy, in the form of University Research Fellowship (URF) to the first author is gratefully acknowledged. The author P.G wishes to acknowledge the University Grant Commission-Basic Scientific Research (UGC-BSR) for the financial assistance provided in the form of one-time grant. The instrumentation facility provided by the University Grant Commission-Special Assistance Program (UGC-SAP) of the Department of Animal Science, Bharathidasan University, is acknowledged.
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Ruban, V.V., Kaliamurthy, J., Dineshkumar, M. et al. Keratitis Due to the Wood Saprobic Ascomycete, Auerswaldia lignicola (Family Botryosphaeriaceae), in a Carpenter in India. Mycopathologia 176, 463–466 (2013). https://doi.org/10.1007/s11046-013-9713-5
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DOI: https://doi.org/10.1007/s11046-013-9713-5