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First Molecular Typing of Cryptococcemia-Causing Cryptococcus in Central-West Brazil

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Abstract

Molecular epidemiology studies on cryptococcemia are limited. This study aimed to describe the clinical features of patients with bloodstream infections by Cryptococcus sp. in a public tertiary hospital in Mato Grosso do Sul, as well as identify the fungus’ molecular type and determine its antifungal susceptibility. Molecular typing was performed using URA5 restriction fragment length polymorphism PCR, and antifungal susceptibility was determined by microdilution method standardized by the Clinical and Laboratory Standards Institute. Over 14 years, 48 patients were diagnosed with cryptococcemia. The majority (72.9 %) was male with a median age of 40 years; 81.3 % of the patients had HIV/AIDS and 72.9 % died. Cryptococcus neoformans was the most commonly isolated species (97.9 %). Molecular analysis identified the genotypes C. neoformans VNI (93.7 %), C. neoformans VNII (4.2 %), and Cryptococcus gattii VGII (2.1 %). In vitro, these fungi were not resistant to fluconazole, itraconazole, voriconazole, and amphotericin B. This is the first description of the molecular types of cryptococcemia agents in central-west Brazil. Its high lethality, especially in HIV-negative patients, suggests that early diagnosis and prompt antifungal therapy are crucial for a good clinical outcome.

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Acknowledgments

The authors thank The HU/UFMS mycology laboratory team for providing Cryptococcus spp. strains. Financial support was from Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior e Pró-Reitoria de Pesquisa e Pós-Graduação da Universidade Federal de Mato Grosso do Sul.

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Authors and Affiliations

  1. Microbiological Research Laboratory, Biology and Health Sciences Center, Federal University of Mato Grosso do Sul, Cidade Universitária, Campo Grande, Mato Grosso do Sul, CEP 79070-900, Brazil

    Rosianne Assis de Sousa Tsujisaki, Débora de Souza Olartechea de Alencar, Fernanda Luíza Espinosa Spositto & Marilene Rodrigues Chang

  2. Medical School, Federal University of Mato Grosso do Sul, Cidade Universitária, Caixa Postal 549, Unidade 9, Campo Grande, Mato Grosso do Sul, CEP 79070-900, Brazil

    Anamaria Mello Miranda Paniago

  3. Federal Institute of Mato Grosso do Sul, 473, km 23, Fazenda Sta. Bárbara, s/n. Caixa Postal 144, Campus Nova Andradina, Nova Andradina, Mato Grosso do Sul, CEP 79750-000, Brazil

    Manoel Sebastião da Costa Lima Júnior

  4. Mycology Laboratory, University Hospital Group, Hospital Universitário Maria Aparecida Pedrossian, Av. Felinto Muller, 355, Campo Grande, Mato Grosso do Sul, CEP 79080-190, Brazil

    Maína de Oliveira Nunes

  5. Laboratory of Mycology, Institute of Clinical Research Evandro Chagas, Fiocruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ, CEP 21040-900, Brazil

    Luciana Trilles

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  1. Rosianne Assis de Sousa Tsujisaki
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  8. Marilene Rodrigues Chang
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Correspondence to Marilene Rodrigues Chang.

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de Sousa Tsujisaki, R.A., Paniago, A.M.M., da Costa Lima Júnior, M.S. et al. First Molecular Typing of Cryptococcemia-Causing Cryptococcus in Central-West Brazil. Mycopathologia 176, 267–272 (2013). https://doi.org/10.1007/s11046-013-9676-6

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  • DOI: https://doi.org/10.1007/s11046-013-9676-6

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