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Antifungal Susceptibility, Exoenzyme Production and Cytotoxicity of Novel Oximes against Candida

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Abstract

Novel oximes were synthesized, their in vitro antifungal activity against Candida was evaluated and their cytotoxicity was determined. The procedure used for the synthesis of the oximes is aligned with the current green chemistry trend; water is employed as the solvent in this reaction. The minimum inhibitory and minimum fungicidal concentrations of the oximes were evaluated using the CLSI M27-A3 method. The influence of these compounds on the inhibition of the production of hydrolytic enzymes, phospholipase and proteinase by Candida was also investigated. The compounds showed a good ability to inhibit phospholipase, with a 50 % reduction in most cases. However, the tested compounds did not affect proteinase. The current results showed a substantial reduction in the phospholipase production, which suggests that compounds of this class may interfere with host infection and disease progression. The oximes examined showed lower fungicidal activities than fluconazole but interfered significantly with the expression of phospholipase. Some of the oximes included in this study could be a suitable matrix for the development of novel antifungal compounds.

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Acknowledgments

The current research was funded by CNPq, CAPES and UFPel.

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Authors and Affiliations

  1. Post-Graduate Programa in Biochemistry and Bioprospection, Federal University of Pelotas, Capão do Leão, RS, Brazil

    J. L. S. Souza, P. H. A. Carvalho & C. M. P. Pereira

  2. Post-Graduate Program in Dentistry, Laboratory of Cell and Tissue Biology (NCTBio), Federal University of Pelotas, Pelotas, RS, Brazil

    F. Nedel & C. M. P. Pereira

  3. Center of Chemical, Pharmaceutical and Food Sciences, Federal University of Pelotas, Pelotas, RS, Brazil

    M. Ritter

  4. Postgraduate Program in Dentistry, School of Dentistry, Federal University of Pelotas (UFPel), Rua Gonçalves Chaves, 457/504, Pelotas, RS, Zip code: 96015-000, Brazil

    R. G. Lund

Authors
  1. J. L. S. Souza
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  2. F. Nedel
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  3. M. Ritter
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  4. P. H. A. Carvalho
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  6. R. G. Lund
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Correspondence to R. G. Lund.

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Souza, J.L.S., Nedel, F., Ritter, M. et al. Antifungal Susceptibility, Exoenzyme Production and Cytotoxicity of Novel Oximes against Candida . Mycopathologia 176, 201–210 (2013). https://doi.org/10.1007/s11046-013-9675-7

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  • DOI: https://doi.org/10.1007/s11046-013-9675-7

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