Mycopathologia

, Volume 175, Issue 5–6, pp 387–397

Analysis of Black Fungal Biofilms Occurring at Domestic Water Taps (I): Compositional Analysis Using Tag-Encoded FLX Amplicon Pyrosequencing

  • Guido Heinrichs
  • Iris Hübner
  • Carsten K. Schmidt
  • G. Sybren de Hoog
  • Gerhard Haase
Article

DOI: 10.1007/s11046-013-9618-3

Cite this article as:
Heinrichs, G., Hübner, I., Schmidt, C.K. et al. Mycopathologia (2013) 175: 387. doi:10.1007/s11046-013-9618-3

Abstract

Mass growth of dark fungal biofilms on water taps and associated habitats was observed in various German drinking water distribution systems recently. Customers of affected drinking water systems are anxious about potential and unknown health risks. These environments are known to harbour a fungal flora also comprising a variety of fungal opportunists that are well known to cause superficial mycoses in humans (Exophiala equina, Exophiala lecanii-corni) but are not known to establish dark biofilms so far. To gain profound insight on composition of respective biofilms, a metagenomic approach using Tag-Encoded FLX Amplicon Pyrosequencing (TEFAP) of the ribosomal internal transcribed spacer 2 region in comparison with a classical cultivation approach using Sabouraud agar with chloramphenicol and erythritol-chloramphenicol-agar was performed. E. lecanii-corni was found to be the major component in 10 of 13 biofilms analysed independently of the method used. Alternaria sp., E. equina, Fusarium spp. and Ochroconis spp. were also relatively abundant. As expected, TEFAP usually revealed a higher diversity than the cultivation approaches. For example, opportunistic species like Candida albicans or Exophiala dermatitidis were detected in very low amounts. In conclusion, TEFAP turned out to be a promising and powerful tool for the semi-quantitative analysis of fungal biofilms. Referring to relevant literature, potential biological hazards caused by fungi of the dark biofilms can be regarded as low.

Keywords

Fungal metagenome Black biofilms Tag-Encoded FLX Amplicon Pyrosequencing Water taps Exophiala lecanii-corni 

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Guido Heinrichs
    • 1
  • Iris Hübner
    • 2
  • Carsten K. Schmidt
    • 2
  • G. Sybren de Hoog
    • 3
    • 4
  • Gerhard Haase
    • 1
  1. 1.Institute of Medical MicrobiologyRWTH Aachen University HospitalAachenGermany
  2. 2.RheinEnergie AGCologneGermany
  3. 3.CBS-KNAW Fungal Biodiversity CentreUtrechtThe Netherlands
  4. 4.Institute of Biodiversity and Ecosystem Dynamics (IBED)AmsterdamThe Netherlands

Personalised recommendations