, Volume 175, Issue 3–4, pp 351–355 | Cite as

Detection of Cryptococcus gattii in Selected Urban Parks of the Willamette Valley, Oregon

  • Jack A. Mortenson
  • Karen H. Bartlett
  • Randy W. Wilson
  • Shawn R. Lockhart


Human and animal infections of the fungus Cryptococcus gattii have been recognized in Oregon since 2006. Transmission is primarily via airborne environmental spores and now thought to be locally acquired due to infection in non-migratory animals and humans with no travel history. Previous published efforts to detect C. gattii from tree swabs and soil samples in Oregon have been unsuccessful. This study was conducted to determine the presence of C. gattii in selected urban parks of Oregon cities within the Willamette Valley where both human and animal cases of C. gattii have been diagnosed. Urban parks were sampled due to spatial and temporal overlap of humans, companion animals and wildlife. Two of 64 parks had positive samples for C. gattii. One park had a positive tree and the other park, 60 miles away, had positive bark mulch samples from a walkway. Genotypic subtypes identified included C. gattii VGIIa and VGIIc, both considered highly virulent in murine host models.


Cryptococcus gattii Oregon Environmental sampling Urban parks Douglas fir tree Bark mulch Pseudotsuga menziesii var. menziesii 



We thank Robert Dyk, Brian Thomas, Trevor Lock, Andrea Mortenson, and Dr. Sara Walker for help with environmental sampling, plus Naureen Iqbal for help with MLST typing. In addition, we thank Darren Bruning and Dr. John Huntley for project design suggestions, Al Shay for tree species identification, Andrew Fox for cartography services, and USDA, APHIS, VS for financial support of the project.


  1. 1.
    Sorrell TC, Chen SC, Ruma P, Meyer W, Pfeiffer TJ, et al. Concordance of clinical and environmental isolates of Cryptococcus neoformans var. gattii by random amplification of polymorphic DNA analysis and PCR fingerprinting. J Clin Microbiol. 1996;34:1253–60.PubMedGoogle Scholar
  2. 2.
    Campbell LT, Currie BJ, Krockenberger M, Malik R, Meyer W, Heitman J, et al. Clonality and recombination in genetically differentiated subgroups of Cryptococcus gattii. Eukaryot Cell. 2005;4:1403–9.PubMedCrossRefGoogle Scholar
  3. 3.
    Stephen C, Lester S, Black W, Fyfe M, Raverty S. Multispecies outbreak of cryptococcosis on southern Vancouver Island, British Columbia. Can Vet J. 2002;43:792–4.PubMedGoogle Scholar
  4. 4.
    MacDougall L, Kidd SE, Galanis E, Mak S, Leslie MJ, Cieslak PR, et al. Spread of Cryptococcus gattii in British Columbia, Canada, and detection in the Pacific Northwest, USA. Emerg Infect Dis. 2007;13:42–50.PubMedCrossRefGoogle Scholar
  5. 5.
    Upton A, Fraser JA, Kidd SE, Bretz C, Bartlett KH, Heitman J, et al. First contemporary case of human infection with Cryptococcus gattii in Puget Sound: evidence for spread of the Vancouver Island outbreak. 2007. J Clin Microbiol. 2007;45:3086–8.PubMedCrossRefGoogle Scholar
  6. 6.
    Byrnes EJ III, Bildfell RJ, Frank SA, Mitchell TG, Marr KA, Heitman J. Molecular evidence that the range of the Vancouver Island outbreak of Cryptococcus gattii infection has expanded into the Pacific Northwest in the United States. J Infect Dis. 2009;199:1081–6.PubMedCrossRefGoogle Scholar
  7. 7.
    Datta K, Bartlett KH, Baer R, Byrnes E, Galanis E, Heitman J, et al. Spread of Cryptococcus gattii into Pacific Northwest region of the United States. Emerg Infect Dis. 2009;15:1185–91.PubMedCrossRefGoogle Scholar
  8. 8.
    Harris JR, Lockhart SR, DeBess E, Marsden-Haug N, Wohrle R, Free C, et al. Clinical aspects of infection due to the emerging pathogen Cryptococcus gattii – United States. Clin Infect Dis. 2011;53:1188–95.PubMedCrossRefGoogle Scholar
  9. 9.
    Kidd SE, Bach PJ, Hingston AO, Mak S, Chow Y, MacDougall L, et al. Cryptococcus gattii dispersal mechanisms, British Columbia, Canada. Emerg Infect Dis. 2007;13:51–7.PubMedCrossRefGoogle Scholar
  10. 10.
    Mak S, Klinkenberg B, Bartlett KH, Fyfe M. Ecological Niche Modeling of Cryptococcus gattii in British Columbia, Canada. Environ Health Perspect. 2010;118:653–8.PubMedCrossRefGoogle Scholar
  11. 11.
    Kidd SE, Chow Y, Mak S, Bach PJ, Chen H, Hingston AO, et al. Characterization of Environmental Sources of the Human and Animal Pathogen Cryptococcus gattii in British Columbia, Canada, and the Pacific Northwest of the United States. Appl Environ Microbiol. 2007;73:1433–43.PubMedCrossRefGoogle Scholar
  12. 12.
    Staib F, Seibold M, Antweiler E, Fröhlich B, Weber S, Blisse A. The brown colour effect (BCE) of Cryptococcus neoformans in the diagnosis, control and epidemiology of C. neoformans infections in AIDS patients. Zentralbl Bakteriol Mikrobiol Hyg A. 1987;266:167–77.PubMedGoogle Scholar
  13. 13.
    Kwon-Chung KJ, Polacheck I, Bennett JE. Improved diagnostic medium for separation of Cryptococcus neoformans var. neoformans (serotypes A and D) and Cryptococcus neoformans var. gattii (serotypes B and C). J Clin Microbiol. 1982;15:535–7.PubMedGoogle Scholar
  14. 14.
    Iqbal N, DeBess EE, Wohrle R, Sun B, Nett RJ, Ahlquis AM, et al. For the Cryptococcus gattii Public Health Working Group. Correlation of genotype and in vitro susceptibilities of Cryptococcus gattii from the Pacific Northwest of the United States. J Clin Microbiol. 2010;48:539–44.PubMedCrossRefGoogle Scholar
  15. 15.
    Meyer W, Aanensen DM, Boekhout T, Cogliati M, Diaz MR, Esposto MC, et al. Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Med Mycol. 2009;47:561–70.PubMedCrossRefGoogle Scholar
  16. 16.
    Fraser JA, Giles SS, Wenink EC, Geunes-Boyer SG, Wright JR, Diezmann S, et al. Same-sex mating and the origin of the Vancouver Island Cryptococcus gattii outbreak. Nature. 2005;437:1360–4.PubMedCrossRefGoogle Scholar
  17. 17.
    Springer DJ, Chaturvedi V. Projecting global occurrence of Cryptococcus gattii. Emerg Infect Dis. 2010;16:114–20.CrossRefGoogle Scholar
  18. 18.
    Bartlett KH, Kidd SE, Kronstad JW. The emergence of Cryptococcus gattii in British Columbia and the Pacific Northwest. Curr Infect Dis Rep. 2008;10:58–65.PubMedCrossRefGoogle Scholar
  19. 19.
    Byrnes EJ III, Li W, Lewit Y, Ma H, Voelz K, Ren P, et al. Emergence and pathogenicity of highly virulent Cryptococcus gattii genotypes in the northwest United States. PLoS Pathog. 2010;6:e1000850.PubMedCrossRefGoogle Scholar

Copyright information

© U.S. Government 2013

Authors and Affiliations

  • Jack A. Mortenson
    • 1
    • 4
  • Karen H. Bartlett
    • 2
  • Randy W. Wilson
    • 1
  • Shawn R. Lockhart
    • 3
  1. 1.USDA, APHIS, Veterinary ServicesSalemUSA
  2. 2.University of British ColumbiaVancouverCanada
  3. 3.Centers for Disease Control and PreventionAtlantaUSA
  4. 4.USDA, Veterinary ServicesTumwaterUSA

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