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Mycopathologia

, Volume 175, Issue 1–2, pp 83–89 | Cite as

Identification of Malassezia Species in Patients with Seborrheic Dermatitis in China

  • Hao Zhang
  • Yuping RanEmail author
  • Zhen Xie
  • Ruifeng Zhang
Article

Abstract

Background

The causes of seborrheic dermatitis (SD) are complex and incompletely understood. Among the factors, Malassezia yeasts have been reported to play a major etiological role in SD. Many previous studies adopted conventional culture methods that were disadvantaged to detect Malassezia microflora in SD patients, resulting in a low detection rate for each species and high variance in types of microflora observed.

Objective

This study analyzed Malassezia microflora in SD patients by applying a transparent dressing to the lesional skin and using direct detection of fungal DNA using nested PCR.

Methods

We collected samples from the lesional skin of 146 SD patients in China and extracted fungal DNA directly from the lesional samples without culture. Specific primers for each Malassezia species were designed to amplify existing yeasts in each sample. Some samples were randomly selected to culture and identified by morphological and physiologic criteria.

Results

M. globosa and M. restricta were found in 87.0 and 81.5 % of seborrheic dermatitis patients, respectively, which together accounted for more than 50 % of Malassezia spp. recovered in these Chinese patients. The majority of SD patients (82.9 %) showed co-colonization of two or more Malassezia species.

Conclusion

M. globosa and M. restricta predominated in Malassezia colonization in Chinese SD patients. Compared with conventional culture, non-culture-based methods may more accurately reflect Malassezia microflora constitution.

Keywords

Malassezia Nested PCR Seborrheic dermatitis 

Notes

Acknowledgments

This work was funded by Project 31100020 and 30570095 of the National Natural Science Foundation of China and supported in part by research grants from Department of Health of Sichuan Province (100141) and scientific research project of North Sichuan Medical College (MP-ZK-34).

Conflict of interest

None.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Hao Zhang
    • 1
    • 2
  • Yuping Ran
    • 1
    Email author
  • Zhen Xie
    • 1
  • Ruifeng Zhang
    • 1
  1. 1.Department of Dermatovenerology, West China HospitalSichuan UniversityChengduPeople’s Republic of China
  2. 2.Department of DermatovenerologyAffiliated Hospital of North Sichuan Medical CollegeNanchongPeople’s Republic of China

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