, Volume 175, Issue 1–2, pp 25–32 | Cite as

Antigens of Coccidioides posadasii as an Important Tool for the Immunodiagnosis of Coccidioidomycosis

  • Rossana de Aguiar CordeiroEmail author
  • Kharla Rabelo Nobre Patoilo
  • Silviane Bandeira Praciano
  • Delia Jessica Astete Medrano
  • Francisca Jakelyne de Farias Marques
  • Liline Maria Soares Martins
  • Kelsen Dantas Eulalio
  • Antônio de Deus Filho
  • Maria do Amparo Salmito Cavalvanti
  • Maria Auxiliadora Bezerra Fechine
  • Raimunda Samia Nogueira Brilhante
  • Zoilo Pires de Camargo
  • Marcos Fábio Gadelha Rocha
  • José Júlio Costa Sidrim


Serologic diagnosis has been presented as a safe alternative for coccidioidomycosis. However, commercial kits based on coccidioidal antibodies available in the USA are considered too expensive for laboratories outside that country. In this study, we describe the preparation of antigens for detection of human coccidioidal antibodies by the immunodiffusion test (ID) and enzyme immunoassay (EIA). Antigens were tested against serum samples from patients with coccidioidomycosis, histoplasmosis and paracoccidioidomycosis, as well as healthy individuals. The highest reactivity in the ID tests was seen in the F0-90 antigen. In the EIAs, the best results were obtained with the F60-90 antigen. None of the serum samples from healthy individuals were recognized by any of the antigen extracts tested by ID or EIA. In conclusion, the F0-90 and F60-90 antigens have the potential to be commercially employed in presumptive diagnosis of coccidioidomycosis by ID or EIA, respectively. The tests could improve serological diagnosis of coccidioidomycosis in South America.


Coccidioidomycosis Diagnosis Antigen Immunodiffusion Enzyme immunoassay 



This work was supported by the National Council for Scientific and Technological Development (CNPq; Brazil; Process: 306637/2010-3 and PRONEX Process 2155-6).


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Rossana de Aguiar Cordeiro
    • 1
    Email author
  • Kharla Rabelo Nobre Patoilo
    • 1
  • Silviane Bandeira Praciano
    • 1
  • Delia Jessica Astete Medrano
    • 1
  • Francisca Jakelyne de Farias Marques
    • 1
  • Liline Maria Soares Martins
    • 2
  • Kelsen Dantas Eulalio
    • 3
  • Antônio de Deus Filho
    • 2
  • Maria do Amparo Salmito Cavalvanti
    • 3
  • Maria Auxiliadora Bezerra Fechine
    • 4
  • Raimunda Samia Nogueira Brilhante
    • 1
  • Zoilo Pires de Camargo
    • 5
  • Marcos Fábio Gadelha Rocha
    • 6
  • José Júlio Costa Sidrim
    • 1
  1. 1.Postgraduate Program in Medical Microbiology, Department of Pathology and Legal MedicineFederal University of CearáFortalezaBrazil
  2. 2.Federal University of PiauíTeresinaBrazil
  3. 3.Instituto de Doenças Tropicais Natan PortellaTeresinaBrazil
  4. 4.Universidade da Integração Internacional da Lusofonia Afro-BrasileiraFortalezaBrazil
  5. 5.Department of Microbiology, Immunology, and ParasitologyFederal University of São PauloSão PauloBrazil
  6. 6.Postgraduate Program in Veterinary ScienceState University of CearáFortalezaBrazil

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