Mycopathologia

, Volume 175, Issue 1–2, pp 153–158 | Cite as

Antifungal Activity of Sodium Bicarbonate Against Fungal Agents Causing Superficial Infections

  • V. Letscher-Bru
  • C. M. Obszynski
  • M. Samsoen
  • M. Sabou
  • J. Waller
  • E. Candolfi
Article

Abstract

Although sodium bicarbonate—NaHCO3 (SB) has many domestic and medical, traditional and empirical uses, only little scientific documentation of its activity is available. The aims of this study were to investigate the antifungal activity of SB on the three fungal groups (yeasts, dermatophytes and molds) responsible for human skin and nail infections. We first evaluated the in vitro antifungal activity of SB on 70 fungal strains isolated from skin and nail infections: 40 dermatophytes, 18 yeasts and 12 molds. A concentration of 10 g/L SB inhibited the growth of 80 % of all the fungal isolates tested on Sabouraud dextrose agar. The minimal inhibitory concentration 90 (MIC90) of SB measured on Sabouraud dextrose agar, Sabouraud dextrose broth and potato dextrose broth was 5 g/L for the yeasts, 20 g/L for the dermatophytes and 40 g/L for the molds. In a second step, we prospectively evaluated the ex vivo antifungal activity of SB on 24 infected (15 dermatophytes, 7 yeasts and 2 molds) clinical specimens (15 nails and 9 skin scrapings). The fungal growth was completely inhibited for 19 (79 %) specimens and reduced for 4 (17 %) specimens after 7 days of incubation on Sabouraud dextrose–chloramphenicol agar supplemented with 10 g/L of SB as compared to Sabouraud dextrose–chloramphenicol agar without SB. In conclusion, we documented the antifungal activity of SB on the most common agents of cutaneous fungal infection and onychomycosis, and we specified the effective concentrations for the different groups of pathogenic fungi. The mechanism of action of SB has yet to be explored.

Keywords

Sodium bicarbonate Dermatophytes Yeasts Molds Onychomycosis Cutaneous fungal infection 

References

  1. 1.
    Schwartz RA. Superficial fungal infections. Lancet. 2004;364:1173–82.PubMedCrossRefGoogle Scholar
  2. 2.
    Welsh O, Vera-Cabrera L, Welsh E. Onychomycosis. Clin Dermatol. 2010;28:151–9.PubMedCrossRefGoogle Scholar
  3. 3.
    Moreno G, Arenas R. Other fungi causing onychomycosis. Clin Dermatol. 2010;28:160–3.Google Scholar
  4. 4.
    Dik AJ, van der Gaag DJ, van Slooten MA. Efficacy of salts against fungal diseases in glasshouse crops. Commun Agric Appl Biol Sci. 2003;68:475–85.PubMedGoogle Scholar
  5. 5.
    Schirra M, D’Aquino S, Palma A, et al. Factors affecting the synergy of thiabendazole, sodium bicarbonate, and heat to control postharvest green mold of citrus fruit. J Agric Food Chem. 2008;56:10793–8.PubMedCrossRefGoogle Scholar
  6. 6.
    Groeschke J, Solassol I, Bressolle F, et al. Stability of amphotericin B and nystatin in antifungal mouthrinses containing sodium hydrogen carbonate. J Pharm Biomed Anal. 2006;42:362–6.PubMedCrossRefGoogle Scholar
  7. 7.
    Patel M, Shackleton JA, Coogan MM, et al. Antifungal effect of mouth rinses on oral Candida counts and salivary flow in treatment-naive HIV-infected patients. AIDS Patient Care STDS. 2008;22:613–8.PubMedCrossRefGoogle Scholar
  8. 8.
    Clinical Laboratory Standards Institute (CLSI). Reference method for broth dilution antifungal susceptibility testing of yeast; approved standard, M27–A2. Wayne: Clinical Laboratory Standards Institute; 2002.Google Scholar
  9. 9.
    Clinical Laboratory Standards Institute (CLSI). Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard, M38-A. Wayne: Clinical Laboratory Standards Institute; 2002.Google Scholar
  10. 10.
    Clinical Laboratory Standards Institute (CLSI). Method for antifungal disk diffusion susceptibility testing in yeasts, approved guideline, M44-A. Wayne: Clinical Laboratory Standards Institute; 2004.Google Scholar
  11. 11.
    Palmer CL, Horst RK, Langhans RW. Use of bicarbonates to inhibit in vitro colony growth of Botrytis cinerea. Plant Dis. 1997;81:1432–8.CrossRefGoogle Scholar
  12. 12.
    Palou L, Smilanick JL, Usall J, et al. Control of postharvest blue and green molds of oranges by hot water, sodium carbonate, and sodium bicarbonate. Plant Dis. 2001;85:371–6.CrossRefGoogle Scholar
  13. 13.
    Rodriguez-Urra AB, Jimenez C, Duenas M, et al. Bicarbonate gradients modulate growth and colony morphology in Aspergillus nidulans. FEMS Microbiol Lett. 2009;300:216–21.PubMedCrossRefGoogle Scholar
  14. 14.
    Jessup CJ, Warner J, Isham N, et al. Antifungal susceptibility testing of dermatophytes: establishing a medium for inducing conidial growth and evaluation of susceptibility of clinical isolates. J Clin Microbiol. 2000;38:341–4.PubMedGoogle Scholar
  15. 15.
    Alio AB, Mendoza M, Zambrano EA, et al. Dermatophytes growth curve and in vitro susceptibility test: a broth micro-titration method. Med Mycol. 2005;43:319–25.PubMedCrossRefGoogle Scholar
  16. 16.
    Ghannoum MA, Chaturvedi V, Espinel-Ingroff A, et al. Intra- and interlaboratory study of a method for testing the antifungal susceptibilities of dermatophytes. J Clin Microbiol. 2004;42:2977–9.PubMedCrossRefGoogle Scholar
  17. 17.
    Verdolini R, Bugatti L, Filosa G, et al. Old fashioned sodium bicarbonate baths for the treatment of psoriasis in the era of futuristic biologics: an old ally to be rescued. J Dermatolog Treat. 2005;16:26–30.PubMedCrossRefGoogle Scholar
  18. 18.
    Montville TJ, Goldstein PK. Sodium bicarbonate reduces viability and alters aflatoxin distribution of Aspergillus parasiticus in Czapek’s agar. Appl Environ Microbiol. 1987;53:2303–7.PubMedGoogle Scholar
  19. 19.
    Corral LG, Post LS, Montville TJ. Antimicrobial activity of sodium bicarbonate. A research note. J Food Sci. 1988;53:981–2.CrossRefGoogle Scholar
  20. 20.
    Curran DM, Montville TJ. Bicarbonate inhibition of Saccharomyces cerevisiae and Hansenula wingei growth in apple juice. Int J Food Microbiol. 1989;8:1–9.PubMedCrossRefGoogle Scholar
  21. 21.
    Aharoni Y, Fallik E, Copel A, et al. Sodium bicarbonate reduces postharvest decay development on melons. Postharvest Biol Technol. 1997;10:201–6.CrossRefGoogle Scholar
  22. 22.
    Smilanick JL, Margosan DA, Mlikota F, et al. Control of citrus green mold by carbonate and bicarbonate salts and the influence of commercial postharvest practices on their efficacy. Plant Dis. 1999;83:139–45.CrossRefGoogle Scholar
  23. 23.
    DePasquale DA, Montville TJ. Mechanism by which ammonium bicarbonate and ammonium sulfate inhibit mycotoxigenic fungi. Appl Environ Microbiol. 1990;56:3711–7.PubMedGoogle Scholar
  24. 24.
    Ko IJ, Kim CW, Houh W, et al. Relationship between Candida albicans producing proteinase (CAPP) and its environmental pH–comparison with a case of trichophyton mentagrophytes. J Korean Med Sci. 1987;2:97–101.PubMedGoogle Scholar
  25. 25.
    Banuelos MA, Sychrova H, Bleykasten-Grosshans C, et al. The Nha1 antiporter of Saccharomyces cerevisiae mediates sodium and potassium efflux. Microbiology. 1998;144(Pt 10):2749–58.PubMedGoogle Scholar
  26. 26.
    Krauke Y, Sychrova H. Four pathogenic candida species differ in salt tolerance. Curr Microbiol. 2010;61:335–9.Google Scholar
  27. 27.
    Page MJ, Di Cera E. Role of Na+ and K+ in enzyme function. Physiol Rev. 2006;86:1049–92.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • V. Letscher-Bru
    • 1
  • C. M. Obszynski
    • 1
  • M. Samsoen
    • 1
  • M. Sabou
    • 1
  • J. Waller
    • 1
  • E. Candolfi
    • 1
  1. 1.Laboratoire de Mycologie Médicale, Institut de Parasitologie et de Pathologie TropicaleCentre Hospitalier Universitaire de StrasbourgStrasbourgFrance

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