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Mycopathologia

, Volume 175, Issue 1–2, pp 135–140 | Cite as

Comparative Evaluation of Borelli’s Lactritmel Agar and Lowenstein-Jensen Agar for Conidiation in the Trichophyton mentagrophytes and Trichophyton rubrum Complexes

  • Aylin Döğen
  • Macit IlkitEmail author
Article

Abstract

The characteristics of macroconidia are the key to the identification of dermatophytic fungi. In this study, we compared Lowenstein-Jensen agar (LJA) with Borelli’s lactritmel agar (BLA) for the ability to induce macroconidia production in a collection of Trichophyton mentagrophytes (12) and Trichophyton rubrum (12) complexes and in Arthroderma isolates (4). We evaluated 28 strains from across the world, which were mainly obtained from the culture collection of the Centraalbureau voor Schimmelcultures, Fungal Biodiversity Centre, The Netherlands. All cultures were incubated at 26 °C on the bench, and conidia formation was investigated every 5 days, over a period of 30 days after inoculation. At 15 days, strains grown on BLA demonstrated better macroconidia production than those grown on LJA, 23 (82.1 %) versus 16 (57.1 %) isolates, respectively. Thus, the use of BLA, an inexpensive medium that can be prepared in the laboratory, should facilitate conidiation and the presumptive identification of dermatophytic fungi in mycology and clinical microbiology laboratories.

Keywords

Dermatophytes Identification Macroconidia Nutrient media Sporulation 

Notes

Acknowledgments

This work was partially supported by the Academic Research Project Units of Çukurova University (project nos: TF2004D7 and TF2010BAP22). We express gratitude to the curators of the CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands, for kindly providing the dermatophyte isolates.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Department of Pharmaceutical Microbiology, Faculty of PharmacyUniversity of MersinMersinTurkey
  2. 2.Division of Mycology, Department of Microbiology, Faculty of MedicineUniversity of ÇukurovaAdanaTurkey

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