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Equine Pythiosis: Report in Crossed Bred (Criole Venezuelan) Horses

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Abstract

Pythium insidiosum is a pathogenic oomycete known since 1890 that causes pythiosis in mammals. In this report, seven P. insidiosum isolates were recovered from Venezuelan horses and were characterized. The strains were recovered from biopsied tissues and kunkers collected from granulomatous masses located on the hind limb and from a nodular lesion in the left upper eyelid, which decrease the ability of the horses to be used for working purposes. The methods used to identify P. insidiosum isolates were based on the production of sporangia and zoospores, histopathology and PCR assay. To further characterize these strains, portions of the 18S rRNA genes of the seven isolates were sequenced. The sequences showed high homology to previously described P. insidiosum DNA sequences available in GenBank. Similar studies based on the morphological, histological and molecular data identified the etiological agent in samples of granulomatous lesions in these equines as P. insidiosum. In America, the infection has been diagnosed more frequently in equines of Brazil, Colombia, Costa Rica and the United States of America.

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Acknowledgments

This work was supported by Research Council (CDCHT) of the Universidad Centroccidental Lisandro Alvarado (project code 009-VE-2008). The authors thank to Dr. Leonel Mendoza for his invaluable help in the scientific reviewing of the manuscript.

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Salas, Y., Márquez, A., Canelón, J. et al. Equine Pythiosis: Report in Crossed Bred (Criole Venezuelan) Horses. Mycopathologia 174, 511–517 (2012). https://doi.org/10.1007/s11046-012-9562-7

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  • DOI: https://doi.org/10.1007/s11046-012-9562-7

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