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Experimental Model of Arthritis Induced by Paracoccidioides brasiliensis in Rats

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Abstract

Paracoccidioidomycosis (PCM), a disease caused by the fungus Paracoccidioides brasiliensis (Pb), is highly prevalent in Brazil, where it is the principal cause of death by systemic mycoses. The disease primarily affects men aged 30–50 year old and usually starts as a pulmonary focus and then may spread to other organs and systems, including the joints. The present study aimed to develop an experimental model of paracoccidioidomycotic arthritis. Two-month-old male Wistar rats (n = 48) were used, divided in 6 groups: test groups EG/15 and EG/45 (received one dose of 100 μl of saline containing 105 Pb viable yeasts in the knee); heat killed Pb-group HK/15 and HK/45 (received a suspension of 105 Pb nonviable yeasts in the knee) and control groups CG/15 and CG/45 (received only sterile saline in the knee). The rats were killed 15 and 45 days postinoculation. In contrast with the control rats, the histopathology of the joints of rats of the test groups (EG/15 and EG/45) revealed a picture of well-established PCM arthritis characterized by extensive sclerosing granulomatous inflammation with numerous multiple budding fungal cells. The X-ray examination revealed joint alterations in these groups. Only metabolic active fungi evoked inflammation. The experimental model was able to induce fungal arthritis in the knees of the rats infected with metabolic active P. brasiliensis. The disease tended to be regressive and restrained by the immune system. No evidence of fungal dissemination to the lungs was observed.

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Acknowledgments

This work was supported by a Grant from Fundação Araucária-Fundo Paraná/SETI and CNPq.

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Authors and Affiliations

  1. Hospital Universitário do Oeste do Paraná, Av. Tancredo Neves, 3224, Bairro: Santo Onofre, CEP 85.806-470, Cascavel, PR, Brazil

    Eduardo Alexandre Loth, Samia Khalil Biazin & Rinaldo Ferreira Gandra

  2. Universidade de São Paulo, São Paulo, Brazil

    Claudete Rodrigues Paula

  3. Universidade Estadual do Oeste do Paraná, Cascavel, Brazil

    Rita de Cássia Garcia Simão

  4. Universidade Federal de São Paulo, São Paulo, Brazil

    Marcello Fabiano de Franco & Rosana Puccia

Authors
  1. Eduardo Alexandre Loth
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  2. Samia Khalil Biazin
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  3. Claudete Rodrigues Paula
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  4. Rita de Cássia Garcia Simão
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  5. Marcello Fabiano de Franco
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  6. Rosana Puccia
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  7. Rinaldo Ferreira Gandra
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Correspondence to Rinaldo Ferreira Gandra.

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Loth, E.A., Biazin, S.K., Paula, C.R. et al. Experimental Model of Arthritis Induced by Paracoccidioides brasiliensis in Rats. Mycopathologia 174, 187–191 (2012). https://doi.org/10.1007/s11046-012-9537-8

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  • DOI: https://doi.org/10.1007/s11046-012-9537-8

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