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First Isolation of Candida dubliniensis from Oral Cavities of Dermatological Patients in Nanjing, China

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Abstract

Candida dubliniensis is an emerging pathogen capable of causing both superficial and systemic infections. Although C. dubliniensis and C. albicans are phenotypically similar, the two species differ in terms of epidemiology and the ability to rapidly develop resistance to fluconazole. C. dubliniensis is primarily associated with oral candidiasis of human immunodeficiency virus (HIV)-infected individuals. In this study, we describe the first recovery of C. dubliniensis from oral cavities of non-HIV-infected patients with dermatological diseases in Nanjing, China. The isolates were phenotypically characterized as C. dubliniensis by their production of brown rough colonies and chlamydospores on tobacco agar and their inability to grow on hypertonic Sabouraud dextrose agar or to assimilate xylose or α-methyl-d-glycoside. The species identification was subsequently confirmed by amplification and sequencing of the internal transcribed spacer region (ITS). Three C. dubliniensis isolates out of 128 (2.3%) presumptive C. albicans/C. dubliniensis ones were finally identified. Further sequence analysis separated the three isolates into two of the four reported ITS genotypes. Antifungal susceptibility testing showed that they were susceptible to fluconazole, itraconazole, voriconazole, micafungin, and amphotericin B. This study adds to the accumulating evidence that C. dubliniensis is widely distributed in non-HIV-infected populations worldwide.

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Authors and Affiliations

  1. Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, 210042, People’s Republic of China

    Yi P. Ge, Gui X. Lu, Yong N. Shen & Wei D. Liu

  2. Department of Rheumatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of China

    Guo X. He

  3. Department of Laser Dermatology, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, People’s Republic of China

    Tong Lin

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  1. Yi P. Ge
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  2. Guo X. He
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  6. Wei D. Liu
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Correspondence to Wei D. Liu.

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Ge, Y.P., He, G.X., Lin, T. et al. First Isolation of Candida dubliniensis from Oral Cavities of Dermatological Patients in Nanjing, China. Mycopathologia 172, 465–471 (2011). https://doi.org/10.1007/s11046-011-9447-1

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  • DOI: https://doi.org/10.1007/s11046-011-9447-1

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