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Comparative Analysis of Extracellular Matrix and Cellular Carbohydrate Expression in the Sporotrichosis and Chromoblastomycosis

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Abstract

This work was based on the analysis of digital images of histochemical profile from subcutaneous lesions in sporotrichosis (ST) and chromoblastomycosis (CM) patients. An additional aim was the detection of carbohydrate expression using lectin histochemical analysis of the different carbohydrates in the fungal cell wall from four different species (Sporothrix schenckii, Fonsecaea pedrosoi, Phialophora verrucosa, and Cladophialophora carrionii) associated with diseases mentioned earlier. Slides from tissue biopsies from ST and CM positive patients (n = 10, each) were stained according to routine techniques. Slides were incubated with 25 μg/ml of Con A lectins and WGA conjugated to peroxidase. Digital image analysis was carried out in a workstation using OPTIMAS™ software system. Routine histochemistry results indicated that there is significantly higher collagen deposition and elastic fibers in ST characteristic lesions compared with that found in CM cases. The ST interstitial fibrosis area was larger than in CM lesions. Comparative lectin binding showed a positive and intense lectin staining pattern in the cell wall of S. schenckii, suggesting a higher expression of glucose/mannose and N-acetyl glucosamine in their cell surface as evidenced by Con A and WGA, respectively. However, these lectins were not effective to recognize some carbohydrates moieties in the F. pedrosoi, P. verrucosa, and C. carrionii. Such findings contribute to additional information about specific recognition processes between fungal parasites and their host cell targets may be mediated by the interaction of carbohydrate-binding proteins, such as lectins, on the surface of one type of cell that combine with complementary sugars on the surface of another cells into fibro-connective tissues associated with lesions.

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Acknowledgments

Authors thank Dr. Rejane Pereira Neves and Dr. Oliane Maria Corrêia Magalhães for their scientific assistance and Dr. William Ledingham for his critical review.

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The authors have no conflict of interest.

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Authors and Affiliations

  1. Laboratório de Imunopatologia Keizo Asami, LIKA, Universidade Federal de Pernambuco, UFPE, Av. Morais Rêgo s/n, Campus Universitário, Recife, PE, 50670-910, Brazil

    Mário Ribeiro de Melo-Júnior & Eduardo Isidoro Carneiro Beltrão

  2. Laboratório de Micologia Médica do Centro de Ciências Biológicas da Universidade Federal de Pernambuco (CCB/UFPE), Recife, Brazil

    Reginaldo Gonçalves de Lima-Neto & Armando Mardsen Lacerda

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  1. Mário Ribeiro de Melo-Júnior
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  2. Reginaldo Gonçalves de Lima-Neto
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  3. Armando Mardsen Lacerda
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  4. Eduardo Isidoro Carneiro Beltrão
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Correspondence to Mário Ribeiro de Melo-Júnior.

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de Melo-Júnior, M.R., de Lima-Neto, R.G., Lacerda, A.M. et al. Comparative Analysis of Extracellular Matrix and Cellular Carbohydrate Expression in the Sporotrichosis and Chromoblastomycosis. Mycopathologia 171, 403–409 (2011). https://doi.org/10.1007/s11046-011-9399-5

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