Abstract
Using a murine model of disseminated infection by two strains of Fusarium verticillioides, we have evaluated the efficacy of high doses of amphotericin B (AMB) (3 mg/kg of body weight/day), voriconazole (VRC) (60 mg/kg of body weight/day), posaconazole (PSC) (100 mg/kg of body weight/day), and the combinations of AMB plus VRC or PSC. In general, our results were very modest. Neither combination was superior to the respective monotherapies. VRC alone and in combination with AMB was able to prolong survival but not to reduce tissue burden, and AMB plus PSC was able to reduce fungal load in organs but not to prolong survival.
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References
Boutati EI, Anaissie EJ. Fusarium, a significant emerging pathogen in patients with hematologic malignancy: ten years’ experience at a cancer center and implications for management. Blood. 1997;90:999–1008.
Nucci M, Anaissie E. Fusarium infections in immunocompromised patients. Clin Microbiol Rev. 2007;20:695–704.
Tortorano AM, Prigitano A, Dho G, Esposto MC, Gianni C, Grancini A, Ossi C, Viviani MA. Species distribution and in vitro antifungal susceptibility patterns of 75 clinical isolates of Fusarium spp. from northern Italy. Antimicrob Agents Chemother. 2008;52:2683–5.
Migheli Q, Balmas V, Harak H, Sanna S, Scherm B, Aoki T, O’Donnell K. Molecular phylogenetic diversity of dermatologic and other human pathogenic fusarial isolates from hospitals in northern and central Italy. J Clin Microbiol. 2010;48:1076–84.
Perfect JR, Marr KA, Walsh TJ, Greenberg RN, DuPont B, De la Torre-Cisneros J, Just-Nübling G, Schlamm HT, Lutsar I, Espinel-Ingroff A, Johnson E. Voriconazole treatment for less-common, emerging, or refractory fungal infections. Clin Infect Dis. 2003;36:1122–31.
Greer ND. Posaconazole (Noxafil): a new triazole antifungal agent. Proc Baiyl Univ Med Cent. 2007; 20:188–96.
Ruíz-Cendoya M, Mariné M, Rodríguez MM, Guarro J. Interactions between triazoles and amphotericin B in treatment of disseminated murine infection by Fusarium oxysporum. Antimicrob Agents Chemother. 2009;53:1705–8.
Ruíz-Cendoya M, Mariné M, Guarro J. Combined therapy in treatment of murine infection by Fusarium solani. J Antimicrob Chemother. 2008;62:543–6.
Ho DY, Lee JD, Rosso F, Montoya JG. Treating disseminated fusariosis: Amphotericin B, voriconazole or both? Mycoses. 2007;50:227–31.
Rodriguez CA, Luján-Zilbermann J, Woodard P, Andreansky M, Adderson EE. Successful treatment of disseminated fusariosis. Bone Marrow Transplant. 2003;31:411–2.
Clinical and Laboratory Standards Institute. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; Approved Standard-Second Edition. CLSI document M38-A2 Wayne, PA: Clinical and Laboratory Standards Institute. 2008.
Eliopoulos GM, Moellering RC. Antimicrobial combinations. In: Lorian V, editor. Antibiotics in laboratory medicine. 3rd ed. Baltimore: The Williams & Wilkins Co; 1991. p. 432–92.
Graybill JR, Bocanegra R, Najvar LK, Loebenberg D, Luther MF. Granulocyte colony-stimulating factor and azole antifungal therapy in murine aspergillosis: Role of immune suppression. Antimicrob Agents Chemother. 1998;42:2467–73.
Clemons KV, Espiritu M, Parmar R, Stevens DA. Comparative efficacies of conventional amphotericin B, liposomal amphotericin B (AmBisome), caspofungin, micafungin, and voriconazole alone and in combination against experimental murine central nervous system aspergillosis. Antimicrob Agents Chemother. 2005;49:4867–75.
Raad II, Hachem RY, Herbrecht R, Graybill JR, Hare R, Corcoran G, Kontoyiannis DP. Posaconazole as salvage treatment for invasive fusariosis in patients with underlying hematologic malignancy and other conditions. Clin Infect Dis. 2006;42:1398–403.
Tezcan G, Ozhak-Baysan B, Alastruey-Izquierdo A, Ogunc D, Ongut G, Yildiran ST, Hazar V, Cuenca-Estrella M, Rodriguez-Tudela JL. Disseminated fusariosis caused by Fusarium verticillioides in an acute lymphoblastic leukemia patient after allogeneic hematopoietic stem cell transplantation. J Clin Microbiol. 2009;47:278–81.
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Ruíz-Cendoya, M., Pastor, J. & Guarro, J. Combined Therapy Against Murine-Disseminated Infection by Fusarium verticillioides . Mycopathologia 171, 171–175 (2011). https://doi.org/10.1007/s11046-010-9364-8
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DOI: https://doi.org/10.1007/s11046-010-9364-8