Abstract
In modern taxonomy, Trichophyton rubrum with the ‘raubitschekii’ morphotype differs from the common strains of T. rubrum morphologically, physiologically, and clinically, despite genetic homogeneity. In this paper, we report the first autochthonous Turkish cases in a 24-year-old woman and her 26-year-old fiancé with tinea cruris and tinea manuum, respectively. The isolates were proven to be T. rubrum with the ‘raubitschekii’ morphotype by their morphological and physiological characteristics including the following: (1) granular colony surface, (2) profuse macroconidia and microconidia, (3) strongly positive urease activity after 3 days, (4) non-perforating hair, (5) restricted growth and producing no alkaline reaction on bromocresol purple-milk solids-glucose agar, and (6) restricted growth and producing no conidia on 3–9% NaCl-supplemented Sabouraud agar. The internal transcribed spacer (ITS) regions of both isolates were sequenced, and the assembled DNA sequences were examined using the Basic BLAST (nucleotide–nucleotide) software of the National Center for Biotechnology Information web database. Actually, the lack of epidemiological data on this variety is notable, and the actual prevalence is unknown. Otherwise, routine methods misidentified the strains as T. rubrum or unrecognized cases were underreported.
References
Kane J, Salkin IF, Weitzman I, Smitka C. Trichophyton raubitschekii sp. nov. Mycotaxon. 1981;13:259–66.
Gräser Y, Kuijpers AF, Presber W, de Hoog GS. Molecular taxonomy of the Trichophyton rubrum complex. J Clin Microbiol. 2000;38:3329–36.
Tietz HJ, Hopp M, Gräser Y. First isolation of Trichophyton raubitschekii (syn. T. rubrum) in Europe. Mycoses. 2002;45:10–4.
Arabatzis M, Velegraki A, Kantardjiev T, et al. First report on autochthonous urease-positive Trichophyton rubrum (T. raubitschekii) from south-east Europe. Br J Dermatol. 2005;153:178–82.
Kane J, Krajden S, Summerbell RC, Sibbald RG. Infections caused by Trichophyton raubitschekii: clinical and epidemiological features. Mycoses. 1990;33:499–506.
Brasch J. Var. raubitschekii of Trichophyton rubrum as a cause of tinea in Germany. Mycoses. 2007;50:2–5.
Ates A, Ozcan K, Ilkit M. Diagnostic value of morphological, physiological and biochemical tests in distinguishing Trichophyton rubrum from Trichophyton mentagrophytes complex. Med Mycol. 2008;46:811–22.
Papini M, Greco C, Pileri F. Onychomycosis caused by an isolate conforming to the description of Trichophyton raubitschekii. Med Mycol. 2004;42:273–6.
Gómez Moyano E, Crespo-Erchiga V, Samaniego González E, del Boz González J, García Martínez S. Tinea cruris (glutealis) de importanción por Trichophyton rubrum var. raubitschekii en España. Rev Iberoam Micol. 2008;25:250–3.
Caiuby MJ, Monteiro PCF, Nishikawa MM. Isolation of Trichophyton raubitschekii in Rio de Janeiro (Brazil). J Med Vet Mycol. 1996;94:361–3.
Lacaz CZ, Zaitz C, Ruiz LR, et al. Dermatophytosis caused by Trichophyton raubitschekii. Report of the first case in São Paulo, Brazil. Rev Inst Med Trop São Paulo. 1999;41:313–7.
Costa AR, Criado PR, Valente NYS, et al. Trichophyton raubitschekii: a new agent of dermatophytosis in Brazil? Dermatol Online J. 2003;9:6.
van Gelderen de Komaid A, Borges de Kestelman I. Urease-positive Trichophyton rubrum strains (previously described as T. raubitschekii): first isolation in Argentina. Eur J Epidemiol. 2001;17:929–33.
Ma L, Li R, Yu J, et al. Majocchi’s granuloma in a liver transplant recipient caused by Trichophyton spp., phenotypically consistent with Trichophyton rubrum var. raubitschekii. Med Mycol. 2009;47:312–6.
Turin L, Riva F, Galbiati G, Cainelli T. Fast, simple and highly sensitive double-rounded polymerase chain reaction assay to detect medically relevant fungi in dermatological specimens. Eur J Clin Invest. 2000;30:511–8.
Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98 NT. Nucl Acids Symp Ser. 1999;41:95–8.
Gómez Moyano E, Crespo-Erchiga V, Hiraldo Gamero A, Segura Palacios JM, García Martínez S. Imported tinea cruris (glutealis) caused by Trichophyton rubrum var. raubitschekii: the third case in Spain. Rev Iberoam Micol. 2010;27:25–6.
Kano R, Nagata M, Suzuki T, et al. Isolation of Trichophyton rubrum var. raubitschekii from a dog. Med Mycol. 2010;48:653–5.
Gupta AK, Ahmad I, Poretta M, Summerbell RC. Arthroconidial formation in Trichophyton raubitschekii. Mycoses. 2003;46:322–8.
Acknowledgments
We gratefully acknowledge Prof. Dr. G. Sybren de Hoog’s (Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands) kind cooperation and confirmation of the isolates examined in this study.
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Ilkit, M., Saraclı, M.A. & Yuksel, T. Trichophyton Rubrum with the ‘raubitschekii’ Morphotype: The First Report from Turkey. Mycopathologia 171, 119–122 (2011). https://doi.org/10.1007/s11046-010-9355-9
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DOI: https://doi.org/10.1007/s11046-010-9355-9