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An Improved Protocol for the Preparation of Total Genomic DNA from Isolates of Yeast and Mould Using Whatman FTA Filter Papers

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Abstract

Here, we present a significantly improved version of our previously published method for the extraction of fungal genomic DNA from pure cultures using Whatman FTA® filter paper matrix technology. This modified protocol is extremely rapid, significantly more cost effective than our original method, and importantly, substantially reduces the problem of potential cross-contamination between sequential filters when employing FTA technology.

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Acknowledgments

We are grateful to Dr John Leeming for his contribution to the development of the methods described here and to the other members of the Mycology Reference Laboratory for their interest and advice.

Conflict of interest statement

AMB has previously received travel grants from Whatman International and Biotage AB, Sweden.

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Authors and Affiliations

  1. Mycology Reference Laboratory, Health Protection Agency, South-West Regional Laboratory, Bristol, UK

    Andrew M. Borman, Mark Fraser, Christopher J. Linton, Michael D. Palmer & Elizabeth M. Johnson

  2. HPA Mycology Reference Laboratory, Myrtle Road, Kingsdown, Bristol, BS2 8EL, UK

    Andrew M. Borman

Authors
  1. Andrew M. Borman
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  2. Mark Fraser
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  3. Christopher J. Linton
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  4. Michael D. Palmer
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  5. Elizabeth M. Johnson
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Corresponding author

Correspondence to Andrew M. Borman.

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Borman, A.M., Fraser, M., Linton, C.J. et al. An Improved Protocol for the Preparation of Total Genomic DNA from Isolates of Yeast and Mould Using Whatman FTA Filter Papers. Mycopathologia 169, 445–449 (2010). https://doi.org/10.1007/s11046-010-9284-7

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  • DOI: https://doi.org/10.1007/s11046-010-9284-7

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