Abstract
Rice and oat flours were analyzed as media for the production of conidia by M. anisopliae var. lepidiotum. The presence of peptone increased conidia yield regardless of the substrate used; however, the highest yield was achieved on oat flour media. The effect of oxygen on conidia production using oat-peptone medium was also studied at two levels: Normal atmosphere (21% O2) and Oxygen-rich pulses (26% O2). Maximum conidia production (4.25 × 107 conidia cm−2) was achieved using 26% O2 pulses after 156 h of culture, which was higher than 100% relative to conidial levels under normal atmosphere. Conidia yield per gram of biomass was 2.6 times higher with 26% O2 (1.12 × 107 conidia mg−1). Conidia quality parameters, such as germination and hydrophobicity, did not show significant differences (P < 0.05) between those treatments. Bioassays parameters, using Tenebrio molitor adults, were analyzed for conidia obtained in both atmospheres and data were fitted to an exponential model. The specific mortality rates were 2.22 and 1.26 days−1, whereas lethal times for 50% mortality were 3.90 and 4.31 days, for 26% O2 pulses and 21% O2 atmosphere, respectively. These results are relevant for production processes since an oxygen increase allowed superior levels of conidia by M. anisopliae without altering quality parameters and virulence toward Tenebrio molitor adults.
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Tlecuitl-Beristain, S. (Reg. No. 117823) thanks the Mexican National Council for Science and Technology (CONACyT) for the scholarship. This study was financed by the Universidad Autónoma Metropolitana- Iztapalapa and Red PROMEP-Mexico. Authors wish to thank Dr. FJ. Fernández for his technical help in the molecular identification of the strain.
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Tlecuitl-Beristain, S., Viniegra-González, G., Díaz-Godínez, G. et al. Medium Selection and Effect of Higher Oxygen Concentration Pulses on Metarhizium anisopliae var. lepidiotum Conidial Production and Quality. Mycopathologia 169, 387–394 (2010). https://doi.org/10.1007/s11046-009-9268-7
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DOI: https://doi.org/10.1007/s11046-009-9268-7