Abstract
An evaluation of the potential hazards associated with mutagenicity and acute toxicity of a mycoherbicide formulation based on the fungal pathogen Cercospora piaropi was performed. Neither the mycoherbicide nor any of its components were mutagenic to Salmonella typhimurium TA98 and TA100 with or without metabolic activation. Both the C. piaropi and the mycoherbicide formulation were shown to be moderately toxic with a bacterial bioluminescence assay. No acute toxicity was found in water samples taken from tanks after treatment of water hyacinth with the mycoherbicide.
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References
Charudattan R. Pathogens for biological control of water hyacinth. In: Charudattan R, Labrada R, Center TD, Kelly-Begazo C, editors. Strategies for water hyacinth control. Report of a panel of experts meeting, 11–14 Sept 1995, Fort Lauderdale, Florida; FAO, Rome, 1996. p. 90–7.
Van thielen R, Ajounou O, Shade V, Neuenschwander O, Aditä A, Lomer CJ. Importation, release and establishment of Neochetina spp (Co.: Curculionidae) for the biological control of water hyacinth Eichhornia crassipes (Lil.: Pontederiaceae), in Benin, west Africa. Enthomophaga. 1994;39:179–88.
Martínez Jiménez M, Charudattan R. Survey and evaluation of Mexican native fungi for potential biocontrol of water hyacinth. J Aquat Plant Manage. 1998;36:145–8.
Martínez Jiménez M, Gutierrez López E. Host range of Cercospora piaropi and Acremonim zonatum, microbial herbicides candidates for water hyacinth. Phytoparasitica. 2001;29(2):175–7.
Ames BN, Lee FD, Durston WE. An improved bacterial test system for the detection and classification of mutagens and carcinogens. Proc Natl Acad Sci USA. 1973;70:782–6. doi:10.1073/pnas.70.3.782.
Ames BN, Durston WE, Yamasaki E, Lee FD. Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection. Proc Natl Acad Sci USA. 1973;70:2281–5. doi:10.1073/pnas.70.8.2281.
Bulich AA, Green MW, Isenbeg DL. Reliability of the bacterial luminescence assay for determination of the toxicity of pure compounds and complex effluents. In: Branson DR, Dickson KL, editors. Aquatic toxicology and hazard assessment. 4th conference ASTM STP 737. American Society of Testing and Materials; 1981.
Ohe T, White PA, De Marini DM. Mutagenic characteristics of river water flowing through large metropolitan areas in North America. Mutat Res. 2003;543:101–12.
Kuczuk MH, Benson PM, Heath H, Hayes AW. Evaluation of the mutagenic potential of mycotoxins using Salmonella typhimurium and Saccharomyces cereviceae. Mutat Res. 1978;53:11–20.
Yates Ida E, Porter JK. Bacterial bioluminescence as bioassay for mycotoxins. Appl Environ Microbiol. 1982;44(5):1072–5.
Sorenson WG, Tucker JD, Simpson JP. Mutagenicity of the tetramic mycotoxin cyclopiazonic acid. Appl Environ Microbiol. 1984;47(6):1355–7.
Jalal MA, Hossain MB, Roberson DJ, van der Helm D. Cercospora beticola phytotoxins: cebetins that are photoactive. Mg2+ binding, chlorinated anthraquinone-xantone conjugates. J Am Chem Soc. 1992;114:5967–71. doi:10.1021/ja00041a009.
Liberman DF, Schaefer FL, Fink RC, Ramgopal M, Ghosh AC, Mulcahy R. Mutagenicity of islandicin and crysophanol in the Salmonella/microsome system. Appl Environ Microbiol. 1980;40(3):476–9.
Daub ME. Cercosporin, a photosensitizing toxin from Cercospora species. Phytopathology. 1982;72(4):370–4.
Yamazaki S, Okube A, Akiyama Y, Fuwa K. Cercosporin, a novel photodynamic pigment isolated from Cercospora kikuchii. Agric Biol Chem. 1975;39:287–8.
Maron MD, Ames NB. Revised methods for the Salmonella mutagenicity test. Mutat Res. 1983;113:173–215.
Mortelmans K, Zeiger E. The Ames Salmonella/microsome mutagenicity assay. Mutat Res. 2000;455:29–60. doi:10.1016/S0027-5107(00)00064-6.
AZUR. ©. Microtox manual, Vol 1–4. Carlsbad: AZUR Environmental (formerly Microbics Corporation); 1998.
Fajola AO. Cercosporin, a phytotoxin from Cercospora species. Physiol Plant Pathol. 1978;13:157–64. doi:10.1016/0048-4059(78)90029-2.
Coleman RN, Quershi AA. Microtox and Spirillum volutans test for assessing toxicity of environmental samples. Bull Environ Contam Toxicol. 1985;35:443–451. doi:10.1007/BF01636536.
Balis C, Payne MG. Triglycerides and cercosporin from Cercospora beticola: fungal growth and cercosporin production. Phytopathology. 1971;61:1477–84.
Milat ML, Prange T, Ducrot PH, Tabet JC, Einhorn J, Blein JP, et al. Structures of the beticolins the yellow toxins produced by Cercospora beticola. J Am Chem Soc. 1992;114:1478–9. doi:10.1021/ja00030a051.
Mitchell Thomas K, Chilton William S, Daub Margaret E. Biodegradation of the polyketide toxin cercosporin. Appl Environ Microbiol. 2002;68(9):4173–81. doi:10.1128/AEM.68.9.4173-4181.2002.
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The authors would like to acknowledge the financial support of SAGARPA-CONACYT (Grant No. 0278). We thank Israel Velasco for statistical assistance.
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Martínez Jiménez, M., Sandoval Villasana, A.M. Evaluation of Toxicity of Cercospora piaropi in a Mycoherbicide Formulation by Using Bacterial Bioluminescence and the Ames Mutagenicity Tests. Mycopathologia 167, 203–208 (2009). https://doi.org/10.1007/s11046-008-9161-9
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DOI: https://doi.org/10.1007/s11046-008-9161-9