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Affinity Purification of Trypsin Inhibitor with Anti-Aspergillus Flavus Activity from Cultivated and Wild Soybean

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Abstract

Trypsin inhibitors (TI) from wild-type soybean (Glycine soya) (WBTI) and domesticated soybean (Glycine max) (SBTI) were purified using prepared chitosan resin-trypsin as filler on the affinity chromatography column. The SBTI/WBTI purification fold by affinity chromatography was 718- and 279-fold, with the activity recovery of 62% and 59%, respectively. It was found that SBTI and WBTI exerted a strong inhibition of Aspergillus. flavus growth, with IC50 of 1.6 and 1.0 μmol/l. This growth inhibition was possibly the result of the inhibition on α-amylase activity of A. flavus by both the SBTI and WBTI. This was further supported by the fact that in the presence of SBTI and WBTI at 9.0 and 6.0 μg/g (peanut) on peanuts inhibited the germination and growth of A. flavus. Accordingly, characterization of the mode of action of SBTI and WBTI could constitute a first step leading to resistance to A. flavus invasion.

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Abbreviations

TI:

Trypsin inhibitor

SBTI:

Soybean (Glycine max) trypsin inhibitor

WBTI:

Wild Soybean (Glycine soya) trypsin inhibitor

A. flavus :

Aspergillus flavus

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Acknowledgement

We thank the Science and Technology Program from Shandong province (2007GG10009005) and the marine project for public benefits from oceanic bureau of China (200805038), and Yan-Li XU for her excellent assistance. Special thanks to Dr. Peter Bucheli from Nestlé R&D Centre Shanghai Ltd.

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Authors and Affiliations

  1. Department of Food Chemistry and Nutrition, College of Food Science and Technology, Ocean University of China, Yu Shan Road 5, Qingdao, 266003, People’s Republic of China

    Bin Zhang, Dong-Feng Wang, Yan Fan, Li Zhang & Yi Luo

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  1. Bin Zhang
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  2. Dong-Feng Wang
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  3. Yan Fan
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  4. Li Zhang
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Correspondence to Dong-Feng Wang.

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Zhang, B., Wang, DF., Fan, Y. et al. Affinity Purification of Trypsin Inhibitor with Anti-Aspergillus Flavus Activity from Cultivated and Wild Soybean. Mycopathologia 167, 163–171 (2009). https://doi.org/10.1007/s11046-008-9160-x

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