Abstract
Purposes
To investigate the early molecular events in oral keratinocytes induced by Candida albicans challenge.
Methods
The oral keratinocyte cell line, Tca8113, was used to study the molecular events induced by C. albicans challenge in oral keratinocytes. The phosphorylation statuses of extracellular signal-regulated protein kinase (ERK) and focal adhesion kinase (FAK) upon C. albicans challenge were assessed using specific antibodies and western blotting. Specific inhibitors for ERK and FAK were used to validate the involvement of ERK-FAK signaling cascade. A Transwell insert system-based migration study was performed to evaluate the involvement of the C. albicans-dependent ERK-FAK activation with cell migration.
Results
Following the stimulation with C. albicans, a transient activation of ERK was observed, which reached a peak at 10 min post stimulation. Similarly, a transient activation of FAK, the downstream substrate of ERK, was also observed upon C. albicans challenges, which reach the maximum at 20 min. Specific inhibitors for ERK and FAK abolished the C. albicans-induced ERK and FAK activations. The elevated migratory ability of oral keratinocyte was observed upon stimulation with C. albicans, and was synchronous with the ERK-FAK activation.
Conclusion
ERK-FAK signaling cascades are involved in the early interaction between the oral keratinocytes and C. albicans, which appears to be linked with the enhanced cell migration.
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Acknowledgments
We acknowledge Dr. Xiaofeng Zhou, at the Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, IL, USA, for his contribution to the language revision, and this work was supported in part by grants from the National Science Funds for Talented Professionals of China (No. 30725041), the National Basic Research Program of China (2008CB517307), the National Natural Science Foundation of China (Nos. 30300387, 30471891, 30672323), New Century Talents Support Program of MOE (NCET-04-0865), Specialized Research Fund for the Doctoral Program of Higher Education (No. 20040610077).
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Jing Shi and Xin Zeng contributed equally to this work.
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Shi, J., Zeng, X., Zhou, M. et al. Activation of ERK-FAK Signaling Pathway and Enhancement of Cell Migration Involved in the Early Interaction Between Oral Keratinocytes and Candida albicans . Mycopathologia 167, 1–7 (2009). https://doi.org/10.1007/s11046-008-9142-z
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DOI: https://doi.org/10.1007/s11046-008-9142-z