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Growth of Phytopathogenic Fungi in the Presence of Partially Acetylated Chitooligosaccharides

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Abstract

Four phytopathogenic fungi were cultivated up to six days in media containing chitooligosaccharide mixtures differing in average DP and F A. The three different mixtures were named Q3 (which contained oligosaccharides of DP2–DP10, with DP2–DP7 as main components), Q2 (which contained oligosaccharides of DP2–DP12, with DP2–DP10 as main components) and Q1 (which derived from Q2 and contained oligomers of DP5–DP8 with hexamer and a heptamer as the main components). The novel aspect of this work is the description of the effect of mixtures of oligosaccharides with different and known composition on fungal growth rates. The growth rate of Alternaria alternata and Rhizopus stolonifer was initially inhibited by Q3 and Q2 at higher concentrations. Q1 had a growth stimulating effect on these two fungi. Growth of Botrytis cinerea was inhibited by Q3 and Q2, while Q1 had no effect on the growth of this fungus. Growth of Penicillium expansum was only slightly inhibited by higher concentrations of sample Q3, while Q2 and Q1 had no effect. The inhibition of growth rates or their resistance toward chitooligosaccharides correlated with the absence or presence of chitinolytic enzymes in the culture media, respectively.

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Abbreviations

A = GlcNAc:

2-Acetamido-2-deoxy-d-glucose

D = GlcN:

2-Amino-2-deoxy-d-glucose

DHB:

2,5-Dihydroxybenzoic acid

DP:

Degree of polymerization

F A :

Mole fraction of GlcNAc residues in chitosan or chitooligosaccharides

GPC:

Gel permeation chromatography

MALDI TOF MS:

Matrix assisted laser desorption ionization time of flight mass spectrometry

GR:

Growth rate

MEA:

Malt extract agar

PDA:

Potato dextrose agar

CM:

Complete medium

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Acknowledgments

Financial support by the Brazilian funding agencies FAPESP, CAPES and CNPq is gratefully acknowledged, as also to the PROBRAL programme (CAPES/DAAD) and to the European Commission (ALFA Programme, II-0259-FA-FC POLYLIFE).

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Authors and Affiliations

  1. School of Chemical Engineering, State University of Campinas, 6066, Campinas, 13081-970, Brazil

    E. N. Oliveira Jr & Telma T. Franco

  2. Institute of Plant Biochemistry and Biotechnology, University of Muenster, Hindenburgplatz 55, 48143, Muenster, Germany

    Nour E. El Gueddari & Bruno M. Moerschbacher

  3. Institute of Chemistry, University of Potsdam, 14415, Potsdam, Germany

    Martin G. Peter

Authors
  1. E. N. Oliveira Jr
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  2. Nour E. El Gueddari
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  3. Bruno M. Moerschbacher
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  4. Martin G. Peter
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  5. Telma T. Franco
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Correspondence to Telma T. Franco.

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Oliveira, E.N., El Gueddari, N.E., Moerschbacher, B.M. et al. Growth of Phytopathogenic Fungi in the Presence of Partially Acetylated Chitooligosaccharides. Mycopathologia 166, 163–174 (2008). https://doi.org/10.1007/s11046-008-9125-0

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