Prevention of Candida albicans biofilm by plant oils
- 723 Downloads
The inhibitory effect of 30 plant oils was evaluated against biofilm forming Candida albicans strain (CA I) isolated from clinical samples, which was sensitive to 4 µg/ml of fluconazole, used as a positive control. The standard strain (MTCC 227, CA II) used in this study was found to be highly resistant to fluconazole, 3,000 µg/ml of which was required to inhibit the growth of this strain partially, and complete inhibition could not be achieved. Eighteen among the 30 plant oils tested were found to show anti-Candida activity by disc diffusion assay. Effective plant oils were assessed using XTT (2, 3-bis [2-Methoxy-4-nitro-5-sulphophenyl]-2H-tetrazolium-5-carboxanilide) reduction assay for biofilm quantification. Four oils eucalyptus, peppermint, ginger grass and clove showed 80.87%, 74.16%, 40.46% and 28.57% biofilm reduction respectively. Minimum inhibitory concentration (MIC) values were calculated using agar dilution assay. Scanning electron microscopic (SEM) analysis further revealed reduction in C. albicans biofilm in response to effective oils. The substantial antifungal activity shown by these plant oils suggests their potential against infections caused by C. albicans.
KeywordsBiofilm Candida albicans Fluconazole Plant oil SEM XTT
Unable to display preview. Download preview PDF.
Authors are thankful to Council for Scientific and Industrial Research (CSIR) and Ministry of␣Human Resource and Development (MHRD), Government of India, for financial support.
- 8.Deans SG. Evaluation of antimicrobial activity of essential (volatile) oils, Modern methods of plant analysis. In: Linskens HF, Jackson JF, editors. Essential oils and waxes, Berlin: Springer Verlag; 1991. p. 309–18.Google Scholar
- 9.Pruthi V, Al-Janabi A, Pereira BMJ. Characterization of biofilm formed on intrauterine devices. Int J Med Microbiol 2003;21:161–5.Google Scholar
- 15.Alviano WS, Mendonca-Filho RR, Alviano DS, Bizzo HR, Souto-Padron T, Rodrigues ML, Bolognese AM, Alviano CS, Souza MMG. Antimicrobial activity of Croton cajucara Benth linalool-rich essential oil on artificial biofilms and planktonic microorganisms. Oral Microbiol Immunol 2005;20:101–5.PubMedCrossRefGoogle Scholar