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Magnesium represses trichothecene biosynthesis and modulates Tri5, Tri6, and Tri12 genes expression in Fusarium graminearum

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Abstract

Growth and production of type-B trichothecenes mycotoxins by the Fusarium graminearum strain CBS 185.32 were compared in GYEP medium supplemented with Mg2+ at different concentrations (0–4 mM). Mg2+ led to a strong decrease in toxin accumulation without affecting the mycelial growth, suggesting a specific Mg2+ effect on fungal secondary metabolism. Expression of Tri5, Tri6, and Tri12 genes was followed throughout the time courses of type-B trichothecenes (TCTB) yield in standard and 2 mM Mg2+-supplemented GYEP media. Mg2+ addition significantly decreased Tri5, Tri6, and Tri12 expression. The inhibition of toxin production by Mg2+ was shown to be highly correlated with inhibition of Tri5 transcription and, to a lesser extend, of Tri6 and Tri12. This is the first report of a transcriptional control of TCTB production by Mg2+.

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Abbreviations

GYEP:

Glucose Yeast Extract Peptone

DON:

Deoxynivalenol

3-ADON:

3-Acetyl-deoxynivalenol

15-ADON:

15-Acetyl-deoxynivalenol

TCTB:

Type-B trichothecenes

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Acknowledgments

We thank Mondher Bouzayen and Thierry Candresse for receiving us in their laboratories for Tri genes experiments.

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Authors and Affiliations

  1. INRA-Mycologie et Sécurité des Aliments, BP 81, 33883, Villenave D’Ornon, France

    Laetitia Pinson-Gadais, Florence Richard-Forget, Christian Barreau, Bernard Cahagnier & Daniel Richard-Molard

  2. INRA-INPT/ENSAT-Unité Génomique et Biotechnologie des Fruits, BP 27, Auzeville, Castanet-Tolosan, 31326, France

    Pierre Frasse

  3. INRA-Unité Protéines Végétales et leurs Interactions, BP 71627, 44316, Nantes, France

    Bénédicte Bakan

Authors
  1. Laetitia Pinson-Gadais
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  2. Florence Richard-Forget
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  3. Pierre Frasse
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  4. Christian Barreau
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  5. Bernard Cahagnier
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  6. Daniel Richard-Molard
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  7. Bénédicte Bakan
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Correspondence to Laetitia Pinson-Gadais.

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Pinson-Gadais, L., Richard-Forget, F., Frasse, P. et al. Magnesium represses trichothecene biosynthesis and modulates Tri5, Tri6, and Tri12 genes expression in Fusarium graminearum . Mycopathologia 165, 51–59 (2008). https://doi.org/10.1007/s11046-007-9076-x

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  • DOI: https://doi.org/10.1007/s11046-007-9076-x

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