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Insights into the pathobiology of Paracoccidioides brasiliensis from transcriptome analysis—advances and perspectives
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  • Published: 24 April 2008

Insights into the pathobiology of Paracoccidioides brasiliensis from transcriptome analysis—advances and perspectives

  • Simoneide S. Silva1,
  • Hugo C. Paes1,
  • Célia M. A. Soares2,
  • Larissa Fernandes1 &
  • …
  • Maria Sueli S. Felipe1 

Mycopathologia volume 165, pages 249–258 (2008)Cite this article

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  • 12 Citations

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Abstract

Paracoccidioiddes brasiliensis is a thermo-dimorphic fungus endemic to Latin America, where it causes the most prevalent systemic mycosis, paracoccidioidomycosis (PCM). DNA microarray technology has been used to identify patterns of gene expression when a microbe is confronted with conditions of interest, such as in vitro and/or ex vivo interaction with specific cells. P. brasiliensis is one organism that has benefited from this approach. Even though its genome has not been sequenced yet, much has been discovered from its transcriptome and DNA array analyses. In this review, we will outline the current knowledge in P.␣brasiliensis transcriptome, with focus on differential expression analysis in vitro and on the discussion of the genes that are controlled during the host–pathogen interaction ex vivo in order to give insights into the pathobiology of this fungus. In vitro experiments enabled the delineation of whole metabolic pathways; the description of differential metabolism between mycelium and yeast cells and of the mainly signaling pathways controlling dimorphism, high temperature growth, thermal and oxidative stress, and virulence/pathogenicity. Recent ex vivo experiments provided advances on the comprehension of the plasticity of response and indicate that P. brasiliensis is not only␣able to undergo fast and dramatic expression profile changes but can also discern subtle differences,␣such as whether it is being attacked by a macrophage or submitted to the bloodstream route conditions.

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Acknowledgments

This work was supported by FAP-DF/CNPq and MCT/CNPq and FUB. SSS, LF and HCP were supported by CNPq fellowship.

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Authors and Affiliations

  1. Laboratório de Biologia Molecular, Departamento de Biologia Celular, Universidade de Brasília, Brasilia, DF, 70910-900, Brazil

    Simoneide S. Silva, Hugo C. Paes, Larissa Fernandes & Maria Sueli S. Felipe

  2. Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Goiás, Goiania, GO, 74001-970, Brazil

    Célia M. A. Soares

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  1. Simoneide S. Silva
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  2. Hugo C. Paes
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  5. Maria Sueli S. Felipe
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Correspondence to Maria Sueli S. Felipe.

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Silva, S.S., Paes, H.C., Soares, C.M.A. et al. Insights into the pathobiology of Paracoccidioides brasiliensis from transcriptome analysis—advances and perspectives. Mycopathologia 165, 249–258 (2008). https://doi.org/10.1007/s11046-007-9071-2

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  • Received: 11 July 2007

  • Accepted: 24 September 2007

  • Published: 24 April 2008

  • Issue Date: April 2008

  • DOI: https://doi.org/10.1007/s11046-007-9071-2

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Keywords

  • Differential gene expression
  • Host–pathogen interaction
  • Macrophage response
  • Paracoccidioides brasiliensis
  • Transcriptome analysis
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